CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis

Hiroaki Obata, Bobby Yanagawa, Koichi Tanaka, Shunsuke Ohnishi, Masaharu Kataoka, Yoshinori Miyahara, Hatsue Ishibashi-Ueda, Makoto Kodama, Yoshifusa Aizawa, Kenji Kangawa, Noritoshi Nagaya

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Myocarditis is an acute inflammatory disease of the myocardium for which there is currently no specific therapy. We investigated the therapeutic potential of C-type natriuretic peptide (CNP) in acute experimental autoimmune myocarditis. One week after injection of porcine myosin into male Lewis rats, CNP (0.05 μg/kg/min) was continuously administered for 2 weeks. CNP infusion significantly increased maximum dP/dt, decreased left ventricular end-diastolic pressure, and improved fractional shortening compared with vehicle administration. In vehicle-treated hearts, severe necrosis and marked infiltration of CD68-positive inflammatory cells were observed. Myocardial and serum levels of monocyte chemoattractant protein-1 were elevated in myocarditis. However, these changes were attenuated by CNP infusion. In addition, treatment with CNP significantly increased myocardial capillary density. Guanylyl cyclase-B, a receptor for CNP, was expressed in myocarditic heart, and cyclic guanosine monophosphate was elevated by CNP infusion. In conclusion, CNP infusion attenuated cardiac function in acute myocarditis through anti-inflammatory and angiogenic effects.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number1
DOIs
Publication statusPublished - 2007 Apr 27
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Myocarditis
Inflammation
Chemokine CCL2
Guanylate Cyclase
Cyclic GMP
Acute Disease
Myosins
Infiltration
Rats
Myocardium
Anti-Inflammatory Agents
Necrosis
Swine
Therapeutics
Blood Pressure
Injections

Keywords

  • Angiogenesis
  • Inflammation
  • MCP-1
  • Myocarditis
  • Natriuretic peptides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Obata, H., Yanagawa, B., Tanaka, K., Ohnishi, S., Kataoka, M., Miyahara, Y., ... Nagaya, N. (2007). CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis. Biochemical and Biophysical Research Communications, 356(1), 60-66. https://doi.org/10.1016/j.bbrc.2007.02.085

CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis. / Obata, Hiroaki; Yanagawa, Bobby; Tanaka, Koichi; Ohnishi, Shunsuke; Kataoka, Masaharu; Miyahara, Yoshinori; Ishibashi-Ueda, Hatsue; Kodama, Makoto; Aizawa, Yoshifusa; Kangawa, Kenji; Nagaya, Noritoshi.

In: Biochemical and Biophysical Research Communications, Vol. 356, No. 1, 27.04.2007, p. 60-66.

Research output: Contribution to journalArticle

Obata, H, Yanagawa, B, Tanaka, K, Ohnishi, S, Kataoka, M, Miyahara, Y, Ishibashi-Ueda, H, Kodama, M, Aizawa, Y, Kangawa, K & Nagaya, N 2007, 'CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis', Biochemical and Biophysical Research Communications, vol. 356, no. 1, pp. 60-66. https://doi.org/10.1016/j.bbrc.2007.02.085
Obata, Hiroaki ; Yanagawa, Bobby ; Tanaka, Koichi ; Ohnishi, Shunsuke ; Kataoka, Masaharu ; Miyahara, Yoshinori ; Ishibashi-Ueda, Hatsue ; Kodama, Makoto ; Aizawa, Yoshifusa ; Kangawa, Kenji ; Nagaya, Noritoshi. / CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 356, No. 1. pp. 60-66.
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