CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis

Hiroaki Obata, Bobby Yanagawa, Koichi Tanaka, Shunsuke Ohnishi, Masaharu Kataoka, Yoshinori Miyahara, Hatsue Ishibashi-Ueda, Makoto Kodama, Yoshifusa Aizawa, Kenji Kangawa, Noritoshi Nagaya

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Abstract

Myocarditis is an acute inflammatory disease of the myocardium for which there is currently no specific therapy. We investigated the therapeutic potential of C-type natriuretic peptide (CNP) in acute experimental autoimmune myocarditis. One week after injection of porcine myosin into male Lewis rats, CNP (0.05 μg/kg/min) was continuously administered for 2 weeks. CNP infusion significantly increased maximum dP/dt, decreased left ventricular end-diastolic pressure, and improved fractional shortening compared with vehicle administration. In vehicle-treated hearts, severe necrosis and marked infiltration of CD68-positive inflammatory cells were observed. Myocardial and serum levels of monocyte chemoattractant protein-1 were elevated in myocarditis. However, these changes were attenuated by CNP infusion. In addition, treatment with CNP significantly increased myocardial capillary density. Guanylyl cyclase-B, a receptor for CNP, was expressed in myocarditic heart, and cyclic guanosine monophosphate was elevated by CNP infusion. In conclusion, CNP infusion attenuated cardiac function in acute myocarditis through anti-inflammatory and angiogenic effects.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume356
Issue number1
DOIs
Publication statusPublished - 2007 Apr 27

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Keywords

  • Angiogenesis
  • Inflammation
  • MCP-1
  • Myocarditis
  • Natriuretic peptides

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Obata, H., Yanagawa, B., Tanaka, K., Ohnishi, S., Kataoka, M., Miyahara, Y., Ishibashi-Ueda, H., Kodama, M., Aizawa, Y., Kangawa, K., & Nagaya, N. (2007). CNP infusion attenuates cardiac dysfunction and inflammation in myocarditis. Biochemical and Biophysical Research Communications, 356(1), 60-66. https://doi.org/10.1016/j.bbrc.2007.02.085