Cockroach GABAB receptor subtypes

Molecular characterization, pharmacological properties and tissue distribution

S. Blankenburg, S. Balfanz, Yoshinobu Hayashi, S. Shigenobu, T. Miura, O. Baumann, A. Baumann, W. Blenau

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

γ-aminobutyric acid (GABA) is the predominant inhibitory neurotransmitter in the central nervous system (CNS). Its effects are mediated by either ionotropic GABAA receptors or metabotropic GABAB receptors. GABAB receptors regulate, via Gi/o G-proteins, ion channels, and adenylyl cyclases. In humans, GABAB receptor subtypes are involved in the etiology of neurologic and psychiatric disorders. In arthropods, however, these members of the G-protein-coupled receptor family are only inadequately characterized. Interestingly, physiological data have revealed important functions of GABAB receptors in the American cockroach, Periplaneta americana. We have cloned cDNAs coding for putative GABAB receptor subtypes 1 and 2 of P. americana (PeaGB1 and PeaGB2). When both receptor proteins are co-expressed in mammalian cells, activation of the receptor heteromer with GABA leads to a dose-dependent decrease in cAMP production. The pharmacological profile differs from that of mammalian and Drosophila GABAB receptors. Western blot analyses with polyclonal antibodies have revealed the expression of PeaGB1 and PeaGB2 in the CNS of the American cockroach. In addition to the widespread distribution in the brain, PeaGB1 is expressed in salivary glands and male accessory glands. Notably, PeaGB1-like immunoreactivity has been detected in the GABAergic salivary neuron 2, suggesting that GABAB receptors act as autoreceptors in this neuron. This article is part of the Special Issue entitled 'GABAergic Signaling in Health and Disease'.

Original languageEnglish
Pages (from-to)134-144
Number of pages11
JournalNeuropharmacology
Volume88
DOIs
Publication statusPublished - 2015 Jan 1
Externally publishedYes

Fingerprint

Periplaneta
Cockroaches
Tissue Distribution
Pharmacology
gamma-Aminobutyric Acid
Central Nervous System
Gi-Go GTP-Binding Protein alpha Subunits
Aminobutyrates
GABAergic Neurons
Autoreceptors
Arthropods
GABA-A Receptors
G-Protein-Coupled Receptors
Salivary Glands
Nervous System Diseases
Ion Channels
Adenylyl Cyclases
Drosophila
Neurotransmitter Agents
Psychiatry

Keywords

  • Adenylyl cyclase
  • Central nervous system
  • G-protein-coupled receptor
  • GABA receptor
  • Periplaneta Americana
  • Salivary gland

ASJC Scopus subject areas

  • Pharmacology
  • Cellular and Molecular Neuroscience

Cite this

Blankenburg, S., Balfanz, S., Hayashi, Y., Shigenobu, S., Miura, T., Baumann, O., ... Blenau, W. (2015). Cockroach GABAB receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution. Neuropharmacology, 88, 134-144. https://doi.org/10.1016/j.neuropharm.2014.08.022

Cockroach GABAB receptor subtypes : Molecular characterization, pharmacological properties and tissue distribution. / Blankenburg, S.; Balfanz, S.; Hayashi, Yoshinobu; Shigenobu, S.; Miura, T.; Baumann, O.; Baumann, A.; Blenau, W.

In: Neuropharmacology, Vol. 88, 01.01.2015, p. 134-144.

Research output: Contribution to journalArticle

Blankenburg, S, Balfanz, S, Hayashi, Y, Shigenobu, S, Miura, T, Baumann, O, Baumann, A & Blenau, W 2015, 'Cockroach GABAB receptor subtypes: Molecular characterization, pharmacological properties and tissue distribution', Neuropharmacology, vol. 88, pp. 134-144. https://doi.org/10.1016/j.neuropharm.2014.08.022
Blankenburg, S. ; Balfanz, S. ; Hayashi, Yoshinobu ; Shigenobu, S. ; Miura, T. ; Baumann, O. ; Baumann, A. ; Blenau, W. / Cockroach GABAB receptor subtypes : Molecular characterization, pharmacological properties and tissue distribution. In: Neuropharmacology. 2015 ; Vol. 88. pp. 134-144.
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