Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy

Susumu Ishida, Kei Shinoda, Shinichi Kawashima, Yoshihisa Oguchi, Yasunori Okada, Eiji Ikeda

Research output: Contribution to journalArticle

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Abstract

PURPOSE. To elucidate vascular endothelial growth factor (VEGF)-mediated pathogenesis of fibrovascular proliferation in diabetic retinopathy. METHODS. Fibrovascular tissues were obtained at vitrectomy from 22 cases with proliferative diabetic retinopathy. The half-divided tissues were processed for reverse transcription-polymerase chain reaction (RT-PCR) analysis to examine the expression of VEGF isoforms and their receptors. Paraffin sections of the other half were used for immunohistochemistry for CD34, glial fibrillary acidic protein and VEGF, and in situ hybridization for VEGF. RESULTS. RT-PCR analysis demonstrated the expression of VEGF receptors VEGF- R1, VEGF-R2, and neuropilin-1 in 12, 14, and 14 of 22 cases, respectively. Notably, VEGF-R2 and neuropilin-1 were simultaneously expressed in the identical 14 tissues. The isoform VEGF121 was constitutively expressed in all the tissues examined, whereas the expression of VEGF165 was confined to the 7 tissues that also expressed VEGF-R2 and neuropilin-1. The vascular density of fibrovascular tissues evaluated by immunohistochemistry for CD34 was significantly higher in the cases with the expression of VEGF-R2 and neuropilin-1 than in those without their expression (P < 0.01), whereas VEGF- R1 expression had no such relationship with the vascular density. The fibrovascular tissues that expressed VEGF165 together with VEGF-R2 and neuropilin-1 were found in significantly younger patients (P < 0.01). In situ hybridization and immunohistochemical studies demonstrated that glial cells in the fibrovascular tissues express and produce VEGF. CONCLUSIONS. Coexpression of VEGF-R2 and neuropilin-1 is suggested to facilitate fibrovascular proliferation in diabetic retinopathy.

Original languageEnglish
Pages (from-to)1649-1656
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number7
Publication statusPublished - 2000

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Neuropilin-1
Vascular Endothelial Growth Factor Receptor
Diabetic Retinopathy
Vascular Endothelial Growth Factor A
Reverse Transcription
In Situ Hybridization
Blood Vessels
Protein Isoforms
Immunohistochemistry
Polymerase Chain Reaction
Glial Fibrillary Acidic Protein
Vitrectomy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ishida, S., Shinoda, K., Kawashima, S., Oguchi, Y., Okada, Y., & Ikeda, E. (2000). Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy. Investigative Ophthalmology and Visual Science, 41(7), 1649-1656.

Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy. / Ishida, Susumu; Shinoda, Kei; Kawashima, Shinichi; Oguchi, Yoshihisa; Okada, Yasunori; Ikeda, Eiji.

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 7, 2000, p. 1649-1656.

Research output: Contribution to journalArticle

Ishida, S, Shinoda, K, Kawashima, S, Oguchi, Y, Okada, Y & Ikeda, E 2000, 'Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy', Investigative Ophthalmology and Visual Science, vol. 41, no. 7, pp. 1649-1656.
Ishida, Susumu ; Shinoda, Kei ; Kawashima, Shinichi ; Oguchi, Yoshihisa ; Okada, Yasunori ; Ikeda, Eiji. / Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy. In: Investigative Ophthalmology and Visual Science. 2000 ; Vol. 41, No. 7. pp. 1649-1656.
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T1 - Coexpression of VEGF receptors VEGF-R2 and neuropilin-1 in proliferative diabetic retinopathy

AU - Ishida, Susumu

AU - Shinoda, Kei

AU - Kawashima, Shinichi

AU - Oguchi, Yoshihisa

AU - Okada, Yasunori

AU - Ikeda, Eiji

PY - 2000

Y1 - 2000

N2 - PURPOSE. To elucidate vascular endothelial growth factor (VEGF)-mediated pathogenesis of fibrovascular proliferation in diabetic retinopathy. METHODS. Fibrovascular tissues were obtained at vitrectomy from 22 cases with proliferative diabetic retinopathy. The half-divided tissues were processed for reverse transcription-polymerase chain reaction (RT-PCR) analysis to examine the expression of VEGF isoforms and their receptors. Paraffin sections of the other half were used for immunohistochemistry for CD34, glial fibrillary acidic protein and VEGF, and in situ hybridization for VEGF. RESULTS. RT-PCR analysis demonstrated the expression of VEGF receptors VEGF- R1, VEGF-R2, and neuropilin-1 in 12, 14, and 14 of 22 cases, respectively. Notably, VEGF-R2 and neuropilin-1 were simultaneously expressed in the identical 14 tissues. The isoform VEGF121 was constitutively expressed in all the tissues examined, whereas the expression of VEGF165 was confined to the 7 tissues that also expressed VEGF-R2 and neuropilin-1. The vascular density of fibrovascular tissues evaluated by immunohistochemistry for CD34 was significantly higher in the cases with the expression of VEGF-R2 and neuropilin-1 than in those without their expression (P < 0.01), whereas VEGF- R1 expression had no such relationship with the vascular density. The fibrovascular tissues that expressed VEGF165 together with VEGF-R2 and neuropilin-1 were found in significantly younger patients (P < 0.01). In situ hybridization and immunohistochemical studies demonstrated that glial cells in the fibrovascular tissues express and produce VEGF. CONCLUSIONS. Coexpression of VEGF-R2 and neuropilin-1 is suggested to facilitate fibrovascular proliferation in diabetic retinopathy.

AB - PURPOSE. To elucidate vascular endothelial growth factor (VEGF)-mediated pathogenesis of fibrovascular proliferation in diabetic retinopathy. METHODS. Fibrovascular tissues were obtained at vitrectomy from 22 cases with proliferative diabetic retinopathy. The half-divided tissues were processed for reverse transcription-polymerase chain reaction (RT-PCR) analysis to examine the expression of VEGF isoforms and their receptors. Paraffin sections of the other half were used for immunohistochemistry for CD34, glial fibrillary acidic protein and VEGF, and in situ hybridization for VEGF. RESULTS. RT-PCR analysis demonstrated the expression of VEGF receptors VEGF- R1, VEGF-R2, and neuropilin-1 in 12, 14, and 14 of 22 cases, respectively. Notably, VEGF-R2 and neuropilin-1 were simultaneously expressed in the identical 14 tissues. The isoform VEGF121 was constitutively expressed in all the tissues examined, whereas the expression of VEGF165 was confined to the 7 tissues that also expressed VEGF-R2 and neuropilin-1. The vascular density of fibrovascular tissues evaluated by immunohistochemistry for CD34 was significantly higher in the cases with the expression of VEGF-R2 and neuropilin-1 than in those without their expression (P < 0.01), whereas VEGF- R1 expression had no such relationship with the vascular density. The fibrovascular tissues that expressed VEGF165 together with VEGF-R2 and neuropilin-1 were found in significantly younger patients (P < 0.01). In situ hybridization and immunohistochemical studies demonstrated that glial cells in the fibrovascular tissues express and produce VEGF. CONCLUSIONS. Coexpression of VEGF-R2 and neuropilin-1 is suggested to facilitate fibrovascular proliferation in diabetic retinopathy.

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