Coffee induces 11β-hydroxysteroid dehydrogenase type 2 activity in human placental choriocarcinoma cells, JEG-3

Tomomi Arao, Hiroomi Tamura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Coffee is the beverage consumed during pregnancy that is most commonly associated with adversely affecting the developing fetoplacental unit. Since placental 11β-hydroxysteroid dehydrogenase type 2 (11β-HSD-2) is an important determinant for maintenance of fetal glucocorticoid levels during pregnancy, we investigated the effect of coffee on 11β-HSD-2 expression in the human placental choriocar-cinoma cell line, JEG-3. Addition of coffee to the culture medium of JEG-3 cells increased 11β-HSD-2 gene expression after 24 hr in a dose-dependent manner. Cellular 11β-HSD-2 activity, which was calculated by the amount of cortisol that was converted to cortisone in the culture medium, also increased in the coffee-treated JEG-3 cells. None of the major constituents of coffee, such as caffeine, caffeic acid or chlorogenic acid, showed any ability to induce 11β-HSD-2 gene expression. The 11β-HSD-2-inducing activity in coffee was extractable with ethyl acetate. Increasing the degree of roasting improved the ability of the coffee extract to induce 11β-HSD-2 gene expression indicating that the active constituent(s) is produced during the roasting process.

Original languageEnglish
Pages (from-to)436-441
Number of pages6
JournalJournal of Health Science
Volume57
Issue number5
DOIs
Publication statusPublished - 2011

Fingerprint

11-beta-Hydroxysteroid Dehydrogenases
Coffee
Choriocarcinoma
Human Activities
Gene expression
Gene Expression
Culture Media
Chlorogenic Acid
Pregnancy
Beverages
Cortisone
Caffeine
Glucocorticoids
Hydrocortisone
Cells
Maintenance
Cell Line

Keywords

  • 11β-hydroxysteroid dehydroge-nase type 2
  • Coffee
  • JEG-3
  • Placenta
  • Trophoblast

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Coffee induces 11β-hydroxysteroid dehydrogenase type 2 activity in human placental choriocarcinoma cells, JEG-3. / Arao, Tomomi; Tamura, Hiroomi.

In: Journal of Health Science, Vol. 57, No. 5, 2011, p. 436-441.

Research output: Contribution to journalArticle

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