Coffee inhibits adipocyte differentiation via inactivation of PPARγ

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Recent epidemiological studies showed that coffee consumption is associated with a lower risk of type 2 diabetes, presumably due to suppression of excess fat accumulation in adipocytes. However, the mechanism underlying the effect of coffee on adipocyte differentiation has not been well documented. To elucidate the mechanism, we investigated the effect of coffee on the differentiation of mouse preadipocyte 3T3-L1 cells. Coffee reduced the accumulation of lipids during adipocytic differentiation of 3T3-L1 cells. At 5% coffee, the accumulation of lipids decreased to half that of the control. Coffee also inhibited the expression of the peroxisome proliferator-activated receptor γ (PPARγ), a transcription factor controlling the differentiation of adipocytes. Furthermore, coffee reduced the expression of other differentiation marker genes, aP2, adiponectin, CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), and lipoprotein lipase (LPL), during adipocyte differentiation. Major bioactive constituents in coffee extracts, such as caffeine, trigonelline, chlorogenic acid, and caffeic acid, showed no effect on PPARγ gene expression. The inhibitory activity was produced by the roasting of the coffee beans.

Original languageEnglish
Pages (from-to)1820-1825
Number of pages6
JournalBiological and Pharmaceutical Bulletin
Volume37
Issue number11
DOIs
Publication statusPublished - 2014 Nov 1

Fingerprint

Peroxisome Proliferator-Activated Receptors
Coffee
Adipocytes
3T3-L1 Cells
CCAAT-Enhancer-Binding Proteins
Lipids
Chlorogenic Acid
Facilitative Glucose Transport Proteins
Lipoprotein Lipase
Adiponectin
Differentiation Antigens
Caffeine
Type 2 Diabetes Mellitus
Epidemiologic Studies
Transcription Factors
Fats
Gene Expression

Keywords

  • 3T3-L1
  • Adipocyte
  • Coffee
  • Differentiation
  • Obesity
  • Peroxisome proliferator-activated receptor γ (PPARγ)

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Coffee inhibits adipocyte differentiation via inactivation of PPARγ. / Aoyagi, Ryohei; Tago, Megumi; Fujiwara, Yosuke; Tamura, Hiroomi.

In: Biological and Pharmaceutical Bulletin, Vol. 37, No. 11, 01.11.2014, p. 1820-1825.

Research output: Contribution to journalArticle

@article{7db803b5ee4f4f45bed2d0565f77573c,
title = "Coffee inhibits adipocyte differentiation via inactivation of PPARγ",
abstract = "Recent epidemiological studies showed that coffee consumption is associated with a lower risk of type 2 diabetes, presumably due to suppression of excess fat accumulation in adipocytes. However, the mechanism underlying the effect of coffee on adipocyte differentiation has not been well documented. To elucidate the mechanism, we investigated the effect of coffee on the differentiation of mouse preadipocyte 3T3-L1 cells. Coffee reduced the accumulation of lipids during adipocytic differentiation of 3T3-L1 cells. At 5{\%} coffee, the accumulation of lipids decreased to half that of the control. Coffee also inhibited the expression of the peroxisome proliferator-activated receptor γ (PPARγ), a transcription factor controlling the differentiation of adipocytes. Furthermore, coffee reduced the expression of other differentiation marker genes, aP2, adiponectin, CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), and lipoprotein lipase (LPL), during adipocyte differentiation. Major bioactive constituents in coffee extracts, such as caffeine, trigonelline, chlorogenic acid, and caffeic acid, showed no effect on PPARγ gene expression. The inhibitory activity was produced by the roasting of the coffee beans.",
keywords = "3T3-L1, Adipocyte, Coffee, Differentiation, Obesity, Peroxisome proliferator-activated receptor γ (PPARγ)",
author = "Ryohei Aoyagi and Megumi Tago and Yosuke Fujiwara and Hiroomi Tamura",
year = "2014",
month = "11",
day = "1",
doi = "10.1248/bpb.b14-00378",
language = "English",
volume = "37",
pages = "1820--1825",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "11",

}

TY - JOUR

T1 - Coffee inhibits adipocyte differentiation via inactivation of PPARγ

AU - Aoyagi, Ryohei

AU - Tago, Megumi

AU - Fujiwara, Yosuke

AU - Tamura, Hiroomi

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Recent epidemiological studies showed that coffee consumption is associated with a lower risk of type 2 diabetes, presumably due to suppression of excess fat accumulation in adipocytes. However, the mechanism underlying the effect of coffee on adipocyte differentiation has not been well documented. To elucidate the mechanism, we investigated the effect of coffee on the differentiation of mouse preadipocyte 3T3-L1 cells. Coffee reduced the accumulation of lipids during adipocytic differentiation of 3T3-L1 cells. At 5% coffee, the accumulation of lipids decreased to half that of the control. Coffee also inhibited the expression of the peroxisome proliferator-activated receptor γ (PPARγ), a transcription factor controlling the differentiation of adipocytes. Furthermore, coffee reduced the expression of other differentiation marker genes, aP2, adiponectin, CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), and lipoprotein lipase (LPL), during adipocyte differentiation. Major bioactive constituents in coffee extracts, such as caffeine, trigonelline, chlorogenic acid, and caffeic acid, showed no effect on PPARγ gene expression. The inhibitory activity was produced by the roasting of the coffee beans.

AB - Recent epidemiological studies showed that coffee consumption is associated with a lower risk of type 2 diabetes, presumably due to suppression of excess fat accumulation in adipocytes. However, the mechanism underlying the effect of coffee on adipocyte differentiation has not been well documented. To elucidate the mechanism, we investigated the effect of coffee on the differentiation of mouse preadipocyte 3T3-L1 cells. Coffee reduced the accumulation of lipids during adipocytic differentiation of 3T3-L1 cells. At 5% coffee, the accumulation of lipids decreased to half that of the control. Coffee also inhibited the expression of the peroxisome proliferator-activated receptor γ (PPARγ), a transcription factor controlling the differentiation of adipocytes. Furthermore, coffee reduced the expression of other differentiation marker genes, aP2, adiponectin, CCAAT-enhancer-binding protein α (C/EBPα), glucose transporter 4 (GLUT4), and lipoprotein lipase (LPL), during adipocyte differentiation. Major bioactive constituents in coffee extracts, such as caffeine, trigonelline, chlorogenic acid, and caffeic acid, showed no effect on PPARγ gene expression. The inhibitory activity was produced by the roasting of the coffee beans.

KW - 3T3-L1

KW - Adipocyte

KW - Coffee

KW - Differentiation

KW - Obesity

KW - Peroxisome proliferator-activated receptor γ (PPARγ)

UR - http://www.scopus.com/inward/record.url?scp=84909587266&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84909587266&partnerID=8YFLogxK

U2 - 10.1248/bpb.b14-00378

DO - 10.1248/bpb.b14-00378

M3 - Article

VL - 37

SP - 1820

EP - 1825

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 11

ER -