Coherent X-ray DIFFRACTION IMAGINg of Cyanidioschyzon merolae

Yuki Sekiguchi, Amane Kobayashi, Yuki Takayama, Mao Oide, Asahi Fukuda, Takahiro Yamamoto, Koji Okajima, Tomotaka Oroguchi, Takeshi Hirakawa, Yayoi Inui, Sachihiro Matsunaga, Masaki Yamamoto, Masayoshi Nakasako

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Coherent X-ray diffraction imaging (CXDI) is a lensless imaging technique for visualizing the structures of noncrystalline particles at a resolution of several tens of nanometers. The targets are particles with dimensions in the sub-micrometer to micrometer range. We carried out CXDI experiments at 66 K to investigate the internal structures of a whole frozen-hydrated cell and a chloroplast of Cyanidioschyzon merolae and a cyanobacteria cell by using coherent X-ray light sources, such as synchrotron and X-ray free-electron laser facilities. Owing to the short wavelength of the X-rays used (0.225 nm), the absorption and multiple scattering of X-rays inside the specimens were negligible. Diffraction patterns from each specimen particle adsorbed onto a thin membrane were collected at resolutions better than 50 μm -1 in reciprocal space. Therefore, structures of specimen particles can be illustrated at a resolution higher than 200 nm in real space. The most probable electron density map was retrieved from each diffraction pattern. Theinternal structures of the specimens are described here, particularly the structural correlation of the chloroplast of C. merolae and the cyanobacteria cell. Based on the experimental results, we discuss the feasibility of CXDI in the structural analyses of biological cells and cellular organelles.

Original languageEnglish
Title of host publicationCyanidioschyzon merolae
Subtitle of host publicationA New Model Eukaryote for Cell and Organelle Biology
PublisherSpringer Singapore
Pages153-173
Number of pages21
ISBN (Electronic)9789811061011
ISBN (Print)9789811061004
DOIs
Publication statusPublished - 2018 Mar 8

Keywords

  • Chloroplast
  • Coherent X-ray diffraction imaging
  • Cyanobacterias
  • Synchrotron radiation
  • Whole-cell imaging
  • X-ray freeelectron laser

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Sekiguchi, Y., Kobayashi, A., Takayama, Y., Oide, M., Fukuda, A., Yamamoto, T., Okajima, K., Oroguchi, T., Hirakawa, T., Inui, Y., Matsunaga, S., Yamamoto, M., & Nakasako, M. (2018). Coherent X-ray DIFFRACTION IMAGINg of Cyanidioschyzon merolae. In Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology (pp. 153-173). Springer Singapore. https://doi.org/10.1007/978-981-10-6101-1_10