Coincidental outbreak of methicillin-resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit

Osamu Imataki, Atsushi Makimoto, Shingo Kato, Takahiro Bannai, Naomi Numa, Yoko Nukui, Yuji Morisawa, Toshihiko Ishida, Masahiro Kami, Takahiro Fukuda, Shin Ichiro Mori, Ryuji Tanosaki, Yoichi Takaue

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common nosocomial pathogens among hospital-acquired infections, and immunocompromised patients are highly susceptive to infection. The molecular typing of isolated strains is a common method for tracing an outbreak of MRSA, but experience with this approach is still limited in the hematopoietic stem cell transplantation (HSCT) ward. Methods: We experienced 6 cases of MRSA infection/colonization in our 26-bed HSCT ward during a 4-week period. This unusual outbreak strongly suggested that the same MRSA strain was involved despite strict isolation and aseptic patient care. Clarification of the transmission pattern was critical, and we applied pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP) assays for evaluation. Results and conclusion: In four of the six cases, the pattern of bands examined by PFGE and AFLP analyses supported the idea that direct person-to-person transmission was very unlikely and the outbreak was coincidental. This experience highlights the clinical value of molecular typing methods for the clinical epidemiological assessment of MRSA outbreak.

Original languageEnglish
Pages (from-to)664-669
Number of pages6
JournalAmerican Journal of Hematology
Volume81
Issue number9
DOIs
Publication statusPublished - 2006 Sep

Fingerprint

Hematopoietic Stem Cell Transplantation
Methicillin-Resistant Staphylococcus aureus
Disease Outbreaks
Molecular Typing
Pulsed Field Gel Electrophoresis
Amplified Fragment Length Polymorphism Analysis
Epidemiologic Methods
Immunocompromised Host
Cross Infection
Infection
Patient Care

Keywords

  • MRSA
  • Outbreak
  • Stem cell transplantation

ASJC Scopus subject areas

  • Hematology

Cite this

Coincidental outbreak of methicillin-resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit. / Imataki, Osamu; Makimoto, Atsushi; Kato, Shingo; Bannai, Takahiro; Numa, Naomi; Nukui, Yoko; Morisawa, Yuji; Ishida, Toshihiko; Kami, Masahiro; Fukuda, Takahiro; Mori, Shin Ichiro; Tanosaki, Ryuji; Takaue, Yoichi.

In: American Journal of Hematology, Vol. 81, No. 9, 09.2006, p. 664-669.

Research output: Contribution to journalArticle

Imataki, O, Makimoto, A, Kato, S, Bannai, T, Numa, N, Nukui, Y, Morisawa, Y, Ishida, T, Kami, M, Fukuda, T, Mori, SI, Tanosaki, R & Takaue, Y 2006, 'Coincidental outbreak of methicillin-resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit', American Journal of Hematology, vol. 81, no. 9, pp. 664-669. https://doi.org/10.1002/ajh.20668
Imataki, Osamu ; Makimoto, Atsushi ; Kato, Shingo ; Bannai, Takahiro ; Numa, Naomi ; Nukui, Yoko ; Morisawa, Yuji ; Ishida, Toshihiko ; Kami, Masahiro ; Fukuda, Takahiro ; Mori, Shin Ichiro ; Tanosaki, Ryuji ; Takaue, Yoichi. / Coincidental outbreak of methicillin-resistant Staphylococcus aureus in a hematopoietic stem cell transplantation unit. In: American Journal of Hematology. 2006 ; Vol. 81, No. 9. pp. 664-669.
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