Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium

Hideyuki Miyashita, Shigeto Shimmura, Hisatoshi Kobayashi, Tetsushi Taguchi, Naoko Asano-Kato, Yuichi Uchino, Masabumi Kato, Jun Shimazaki, Junzo Tanaka, Kazuo Tsubota

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The cornea is a transparent tissue of the eye, which is responsible for the refraction of incoming light. Both biological corneal equivalents and synthetic keratoprostheses have been developed to replace donor tissue as a means to restore vision. However, both designs have drawbacks in terms of stability and biocompatibility. Clinically available synthetic devices do not support an intact epithelium, which poses a risk of microbial infection or protrusion of the prosthesis. In the present study, type I collagen was immobilized onto poly(vinyl alcohol) (PVA-COL) as a possible artificial cornea scaffold that can sustain a functional corneal epithelium. Human and rabbit corneal epithelial cells were air-lift cultured with 3T3 feeder fibroblasts to form a stratified epithelial layer on PVA-COL. The epithelial sheet expressed keratin 3/12 differentiation markers, the tight junction protein occludin, and had characteristic microvilli structures on transmission electron microscopy. Functionally, the stratified epithelium contained normal glycogen levels, and an apical tight-junction network was observed to exclude the diffusion of horseradish peroxidase. Furthermore, the epithelium-PVA-COL composite was suturable in the rabbit cornea, suggesting the possibility of using PVA-COL as a biocompatible material for keratoprosthesis.

Original languageEnglish
Pages (from-to)56-63
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume76
Issue number1
DOIs
Publication statusPublished - 2006 Jan

Fingerprint

Collagen
Scaffolds
Alcohols
Keratin-3
Tissue
Occludin
Tight Junction Proteins
Keratin
Differentiation Antigens
Biocompatible Materials
Horseradish Peroxidase
Fibroblasts
Collagen Type I
Refraction
Glycogen
Biocompatibility
Biomaterials
Transmission electron microscopy
Proteins
Composite materials

Keywords

  • Collagen
  • Cornea
  • Epithelium
  • Keratoprosthesis
  • Poly(vinyl alcohol)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium. / Miyashita, Hideyuki; Shimmura, Shigeto; Kobayashi, Hisatoshi; Taguchi, Tetsushi; Asano-Kato, Naoko; Uchino, Yuichi; Kato, Masabumi; Shimazaki, Jun; Tanaka, Junzo; Tsubota, Kazuo.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 76, No. 1, 01.2006, p. 56-63.

Research output: Contribution to journalArticle

Miyashita, Hideyuki ; Shimmura, Shigeto ; Kobayashi, Hisatoshi ; Taguchi, Tetsushi ; Asano-Kato, Naoko ; Uchino, Yuichi ; Kato, Masabumi ; Shimazaki, Jun ; Tanaka, Junzo ; Tsubota, Kazuo. / Collagen-immobilized poly(vinyl alcohol) as an artificial cornea scaffold that supports a stratified corneal epithelium. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2006 ; Vol. 76, No. 1. pp. 56-63.
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