Abstract
We present a perfusable collagen-silicone hybrid microtube device for three-dimensional (3D) tissue culture mimicking microenvironment in vivo. Our device is composed of a collagen hydrogel microtube that are directly connected to two silicone tubes. In the collagen microtube, multiple types of cells can be cultured in 3D extracellular matrix (ECM) microenvironment with perfusion. The connected silicone tubes enable us to connect an external pump system easily. We confirmed that cells can be cultured in our device and perfusion assay can be performed by testing the alignment of actin filaments in endothelial cells by fluid shear stress. Our collagen tube device could be a simple but easy-to-use in vitro perfusable 3D tissue models for drug testing and regenerative medicine.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 419-420 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 16/10/9 → 16/10/13 |
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Keywords
- Cell culture
- Collagen
- Endothelial cells
- Microtube
- Perfusion
ASJC Scopus subject areas
- Control and Systems Engineering
Cite this
Collagen-silicone hybrid microtube device for 3D-layered tissue culture with perfusion. / Tajima, H.; Onoe, Hiroaki.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 419-420.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Collagen-silicone hybrid microtube device for 3D-layered tissue culture with perfusion
AU - Tajima, H.
AU - Onoe, Hiroaki
PY - 2016
Y1 - 2016
N2 - We present a perfusable collagen-silicone hybrid microtube device for three-dimensional (3D) tissue culture mimicking microenvironment in vivo. Our device is composed of a collagen hydrogel microtube that are directly connected to two silicone tubes. In the collagen microtube, multiple types of cells can be cultured in 3D extracellular matrix (ECM) microenvironment with perfusion. The connected silicone tubes enable us to connect an external pump system easily. We confirmed that cells can be cultured in our device and perfusion assay can be performed by testing the alignment of actin filaments in endothelial cells by fluid shear stress. Our collagen tube device could be a simple but easy-to-use in vitro perfusable 3D tissue models for drug testing and regenerative medicine.
AB - We present a perfusable collagen-silicone hybrid microtube device for three-dimensional (3D) tissue culture mimicking microenvironment in vivo. Our device is composed of a collagen hydrogel microtube that are directly connected to two silicone tubes. In the collagen microtube, multiple types of cells can be cultured in 3D extracellular matrix (ECM) microenvironment with perfusion. The connected silicone tubes enable us to connect an external pump system easily. We confirmed that cells can be cultured in our device and perfusion assay can be performed by testing the alignment of actin filaments in endothelial cells by fluid shear stress. Our collagen tube device could be a simple but easy-to-use in vitro perfusable 3D tissue models for drug testing and regenerative medicine.
KW - Cell culture
KW - Collagen
KW - Endothelial cells
KW - Microtube
KW - Perfusion
UR - http://www.scopus.com/inward/record.url?scp=85014186866&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014186866&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014186866
SP - 419
EP - 420
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
ER -