Collagen-silicone hybrid microtube device for 3D-layered tissue culture with perfusion

H. Tajima, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a perfusable collagen-silicone hybrid microtube device for three-dimensional (3D) tissue culture mimicking microenvironment in vivo. Our device is composed of a collagen hydrogel microtube that are directly connected to two silicone tubes. In the collagen microtube, multiple types of cells can be cultured in 3D extracellular matrix (ECM) microenvironment with perfusion. The connected silicone tubes enable us to connect an external pump system easily. We confirmed that cells can be cultured in our device and perfusion assay can be performed by testing the alignment of actin filaments in endothelial cells by fluid shear stress. Our collagen tube device could be a simple but easy-to-use in vitro perfusable 3D tissue models for drug testing and regenerative medicine.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages419-420
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

Keywords

  • Cell culture
  • Collagen
  • Endothelial cells
  • Microtube
  • Perfusion

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Tajima, H., & Onoe, H. (2016). Collagen-silicone hybrid microtube device for 3D-layered tissue culture with perfusion. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 419-420). Chemical and Biological Microsystems Society.