Colony-Stimulating Factor 1 Expression Is Down-Regulated during the Adipocyte Differentiation of H-1/A Marrow Stromal Cells and Induced by Cachectin/Tumor Necrosis Factor

Akihiro Umezawa, Kouichi Tachibana, Kenichi Harigaya, Satoshi Kusakari, Shingo Kato, Yonosuke Watanabe, Toshiya Takano

Research output: Contribution to journalArticle

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Abstract

We isolated clonal sublines of the established mouse marrow stromal cell line, H-1. These clonal sublines underwent differentiation into adipocytes in various degrees. One subline, H-1/A, underwent adipocyte differentiation after confluence, while another subline, H-1/D, did not differentiate. In H-1/A cells, the 4.5- and 2.5-kb major mRNA species of colony-stimulating factor 1 (CSF-1) were expressed before differentiation and were down-regulated at a posttranscriptional level during the differentiation of H-1/A cells. The downregulation of the CSF-1 gene was not a result of arrested cellular growth, because no down-regulation was detected in the nondifferentiating sister line, H-1/D. This down-regulation appeared to be an early event in differentiation. Cachectin/tumor necrosis factor transiently induced the expression of CSF-1 and inhibited the differentiation of H-1/A cells into adipocytes. This induced expression of CSF-1 was due to an increased rate of transcription.

Original languageEnglish
Pages (from-to)920-927
Number of pages8
JournalMolecular and Cellular Biology
Volume11
Issue number2
Publication statusPublished - 1991 Feb

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Macrophage Colony-Stimulating Factor
Stromal Cells
Adipocytes
Tumor Necrosis Factor-alpha
Bone Marrow
Down-Regulation
Cell Line
Messenger RNA
Growth
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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Colony-Stimulating Factor 1 Expression Is Down-Regulated during the Adipocyte Differentiation of H-1/A Marrow Stromal Cells and Induced by Cachectin/Tumor Necrosis Factor. / Umezawa, Akihiro; Tachibana, Kouichi; Harigaya, Kenichi; Kusakari, Satoshi; Kato, Shingo; Watanabe, Yonosuke; Takano, Toshiya.

In: Molecular and Cellular Biology, Vol. 11, No. 2, 02.1991, p. 920-927.

Research output: Contribution to journalArticle

Umezawa, Akihiro ; Tachibana, Kouichi ; Harigaya, Kenichi ; Kusakari, Satoshi ; Kato, Shingo ; Watanabe, Yonosuke ; Takano, Toshiya. / Colony-Stimulating Factor 1 Expression Is Down-Regulated during the Adipocyte Differentiation of H-1/A Marrow Stromal Cells and Induced by Cachectin/Tumor Necrosis Factor. In: Molecular and Cellular Biology. 1991 ; Vol. 11, No. 2. pp. 920-927.
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