Colony-stimulating factor 1 expression is down-regulated during the adipocyte differentiation of H-1/A marrow stromal cells and induced by cachectin/tumor necrosis factor

A. Umezawa, K. Tachibana, K. Harigaya, S. Kusakari, S. Kato, Y. Watanabe, T. Takano

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46 Citations (Scopus)

Abstract

We isolated clonal sublines of the established mouse marrow stromal cell line, H-1. These clonal sublines underwent differentiation into adipocytes in various degrees. One subline, H-1/A, underwent adipocyte differentiation after confluence, while another subline, H-1/D, did not differentiate. In H-1/A cells, the 4.5- and 2.5-kb major mRNA species of colony-stimulating factor 1 (CSF-1) were expressed before differentiation and were down-regulated at a posttranscriptional level during the differentiation of H-1/A cells. The down-regulation of the CSF-1 gene was not a result of arrested cellular growth, because no down-regulation was detected in the nondifferentiating sister line, H-1/D. This down-regulation appeared to be an early event in differentiation. Cachectin/tumor necrosis factor transiently induced the expression of CSF-1 and inhibited the differentiation of H-1/A cells into adipocytes. The induced expression of CSF-1 was due to an increased rate of transcription.

Original languageEnglish
Pages (from-to)920-927
Number of pages8
JournalMolecular and cellular biology
Volume11
Issue number2
DOIs
Publication statusPublished - 1991

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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