Common epitopes on the SS-B/La proteins of 41 and 29 kD recognized by human autoantibodies

T. Okano, M. Akizuki, H. Yamagata, Y. Ohsone, Michito Hirakata, M. Satoh, M. Homma

Research output: Contribution to journalArticle

Abstract

The calf thymus SS-B/La antigen precipitated by specific antibodies or purified by affinity chromatography has two major antigenic proteins. The proteins of 41 and 29 kD were found whether whole calf thymus extract (CTE) or the 60-80% saturated ammonium sulfate fraction of CTE were used as a source of SS-B/La. Both proteins were shown to react with human autoantibodies to SS-B/La by immunoblotting. Purified anti-41 and 29 kD SS-B/La antibodies reacted with both 29 and 41 kD proteins in immunoblotting. In agar diffusion, purified anti-41 and 29 kD SS-B/La antibodies and anti-SS-B/La antibodies in the original serum showed a reaction of identity. Both anti-41 and 29 kD protein antibodies showed three immunofluorescent staining patterns on rat liver cells: speckled; nucleolar and speckled associated with disperse cytoplasmic. These results indicate that the common antigenic determinants present on the 41 and 29 kD SS-B/La proteins are recognized by human autoantibodies to the SS-B/La antigen produced in connective tissue diseases.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalJapanese Journal of Rheumatology
Volume2
Issue number1
Publication statusPublished - 1989
Externally publishedYes

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Autoantibodies
Epitopes
Thymus Extracts
Proteins
Immunoblotting
Connective Tissue Diseases
Antibodies
Ammonium Sulfate
Affinity Chromatography
Thymus Gland
Agar
IgA receptor
Staining and Labeling
SS-B antibodies
Liver
Serum
SS-B antigen

ASJC Scopus subject areas

  • Rheumatology

Cite this

Okano, T., Akizuki, M., Yamagata, H., Ohsone, Y., Hirakata, M., Satoh, M., & Homma, M. (1989). Common epitopes on the SS-B/La proteins of 41 and 29 kD recognized by human autoantibodies. Japanese Journal of Rheumatology, 2(1), 1-12.

Common epitopes on the SS-B/La proteins of 41 and 29 kD recognized by human autoantibodies. / Okano, T.; Akizuki, M.; Yamagata, H.; Ohsone, Y.; Hirakata, Michito; Satoh, M.; Homma, M.

In: Japanese Journal of Rheumatology, Vol. 2, No. 1, 1989, p. 1-12.

Research output: Contribution to journalArticle

Okano, T, Akizuki, M, Yamagata, H, Ohsone, Y, Hirakata, M, Satoh, M & Homma, M 1989, 'Common epitopes on the SS-B/La proteins of 41 and 29 kD recognized by human autoantibodies', Japanese Journal of Rheumatology, vol. 2, no. 1, pp. 1-12.
Okano, T. ; Akizuki, M. ; Yamagata, H. ; Ohsone, Y. ; Hirakata, Michito ; Satoh, M. ; Homma, M. / Common epitopes on the SS-B/La proteins of 41 and 29 kD recognized by human autoantibodies. In: Japanese Journal of Rheumatology. 1989 ; Vol. 2, No. 1. pp. 1-12.
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AU - Akizuki, M.

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AU - Hirakata, Michito

AU - Satoh, M.

AU - Homma, M.

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N2 - The calf thymus SS-B/La antigen precipitated by specific antibodies or purified by affinity chromatography has two major antigenic proteins. The proteins of 41 and 29 kD were found whether whole calf thymus extract (CTE) or the 60-80% saturated ammonium sulfate fraction of CTE were used as a source of SS-B/La. Both proteins were shown to react with human autoantibodies to SS-B/La by immunoblotting. Purified anti-41 and 29 kD SS-B/La antibodies reacted with both 29 and 41 kD proteins in immunoblotting. In agar diffusion, purified anti-41 and 29 kD SS-B/La antibodies and anti-SS-B/La antibodies in the original serum showed a reaction of identity. Both anti-41 and 29 kD protein antibodies showed three immunofluorescent staining patterns on rat liver cells: speckled; nucleolar and speckled associated with disperse cytoplasmic. These results indicate that the common antigenic determinants present on the 41 and 29 kD SS-B/La proteins are recognized by human autoantibodies to the SS-B/La antigen produced in connective tissue diseases.

AB - The calf thymus SS-B/La antigen precipitated by specific antibodies or purified by affinity chromatography has two major antigenic proteins. The proteins of 41 and 29 kD were found whether whole calf thymus extract (CTE) or the 60-80% saturated ammonium sulfate fraction of CTE were used as a source of SS-B/La. Both proteins were shown to react with human autoantibodies to SS-B/La by immunoblotting. Purified anti-41 and 29 kD SS-B/La antibodies reacted with both 29 and 41 kD proteins in immunoblotting. In agar diffusion, purified anti-41 and 29 kD SS-B/La antibodies and anti-SS-B/La antibodies in the original serum showed a reaction of identity. Both anti-41 and 29 kD protein antibodies showed three immunofluorescent staining patterns on rat liver cells: speckled; nucleolar and speckled associated with disperse cytoplasmic. These results indicate that the common antigenic determinants present on the 41 and 29 kD SS-B/La proteins are recognized by human autoantibodies to the SS-B/La antigen produced in connective tissue diseases.

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