Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells

Yoshiaki Tabuchi, Yukihiro Furusawa, Ayako Kariya, Shigehito Wada, Kenzo Ohtsuka, Takashi Kondo

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Heat stress induces complex cellular responses, and its detailed molecular mechanisms still remain to be clarified. The objective of this study was to investigate the molecular mechanisms underlying cellular responses to mild hyperthermia (MHT) in normal human fibroblastic (NHF) cells. Materials and methods: Cells were treated with MHT (41°C, 30min) and then cultured at 37°C. Gene expression was determined by the GeneChip® system and bioinformatics tools. Results: Treatment of the NHF cell lines, Hs68 and OUMS-36, with MHT did not affect the cell viability or cell cycle. In contrast, many probe sets were differentially expressed by >1.5-fold in both cell lines after MHT treatment. Of the 1,196 commonly and differentially expressed probe sets analysed by k-means clustering, three gene clusters, Up-I, Down-I and Down-II, were observed. Interestingly, two gene networks were obtained from the up-regulated genes in cluster Up-I. The gene network E contained DDIT3 and HSPA5 and was mainly associated with the biological process of endoplasmic reticulum stress, while the network S contained HBEGF and LIF and was associated with the biological process of cell survival. Eighteen genes were validated by quantitative real-time polymerase chain reaction, consistent with the microarray data, in four kinds of NHF cells. Conclusions: Common genes that were differentially expressed and/or acted within a gene network in response to MHT in NHF cells were identified. These findings provide the molecular basis for a further understanding of the mechanisms of the MHT response in NHF cells.

Original languageEnglish
Pages (from-to)38-50
Number of pages13
JournalInternational Journal of Hyperthermia
Volume29
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Fever
Gene Expression
Temperature
Gene Regulatory Networks
Biological Phenomena
Multigene Family
Cell Survival
Cell Line
Endoplasmic Reticulum Stress
Computational Biology
Genes
Cluster Analysis
Real-Time Polymerase Chain Reaction
Cell Cycle
Hot Temperature

Keywords

  • gene function
  • gene network
  • microarray
  • Mild temperature hyperthermia
  • normal human fibroblastic cell

ASJC Scopus subject areas

  • Cancer Research
  • Physiology
  • Radiological and Ultrasound Technology
  • Physiology (medical)

Cite this

Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. / Tabuchi, Yoshiaki; Furusawa, Yukihiro; Kariya, Ayako; Wada, Shigehito; Ohtsuka, Kenzo; Kondo, Takashi.

In: International Journal of Hyperthermia, Vol. 29, No. 1, 2013, p. 38-50.

Research output: Contribution to journalArticle

Tabuchi, Yoshiaki ; Furusawa, Yukihiro ; Kariya, Ayako ; Wada, Shigehito ; Ohtsuka, Kenzo ; Kondo, Takashi. / Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells. In: International Journal of Hyperthermia. 2013 ; Vol. 29, No. 1. pp. 38-50.
@article{aa0958cebf3847ea91484ab07a091202,
title = "Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells",
abstract = "Purpose: Heat stress induces complex cellular responses, and its detailed molecular mechanisms still remain to be clarified. The objective of this study was to investigate the molecular mechanisms underlying cellular responses to mild hyperthermia (MHT) in normal human fibroblastic (NHF) cells. Materials and methods: Cells were treated with MHT (41°C, 30min) and then cultured at 37°C. Gene expression was determined by the GeneChip{\circledR} system and bioinformatics tools. Results: Treatment of the NHF cell lines, Hs68 and OUMS-36, with MHT did not affect the cell viability or cell cycle. In contrast, many probe sets were differentially expressed by >1.5-fold in both cell lines after MHT treatment. Of the 1,196 commonly and differentially expressed probe sets analysed by k-means clustering, three gene clusters, Up-I, Down-I and Down-II, were observed. Interestingly, two gene networks were obtained from the up-regulated genes in cluster Up-I. The gene network E contained DDIT3 and HSPA5 and was mainly associated with the biological process of endoplasmic reticulum stress, while the network S contained HBEGF and LIF and was associated with the biological process of cell survival. Eighteen genes were validated by quantitative real-time polymerase chain reaction, consistent with the microarray data, in four kinds of NHF cells. Conclusions: Common genes that were differentially expressed and/or acted within a gene network in response to MHT in NHF cells were identified. These findings provide the molecular basis for a further understanding of the mechanisms of the MHT response in NHF cells.",
keywords = "gene function, gene network, microarray, Mild temperature hyperthermia, normal human fibroblastic cell",
author = "Yoshiaki Tabuchi and Yukihiro Furusawa and Ayako Kariya and Shigehito Wada and Kenzo Ohtsuka and Takashi Kondo",
year = "2013",
doi = "10.3109/02656736.2012.753163",
language = "English",
volume = "29",
pages = "38--50",
journal = "International Journal of Hyperthermia",
issn = "0265-6736",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Common gene expression patterns responsive to mild temperature hyperthermia in normal human fibroblastic cells

AU - Tabuchi, Yoshiaki

AU - Furusawa, Yukihiro

AU - Kariya, Ayako

AU - Wada, Shigehito

AU - Ohtsuka, Kenzo

AU - Kondo, Takashi

PY - 2013

Y1 - 2013

N2 - Purpose: Heat stress induces complex cellular responses, and its detailed molecular mechanisms still remain to be clarified. The objective of this study was to investigate the molecular mechanisms underlying cellular responses to mild hyperthermia (MHT) in normal human fibroblastic (NHF) cells. Materials and methods: Cells were treated with MHT (41°C, 30min) and then cultured at 37°C. Gene expression was determined by the GeneChip® system and bioinformatics tools. Results: Treatment of the NHF cell lines, Hs68 and OUMS-36, with MHT did not affect the cell viability or cell cycle. In contrast, many probe sets were differentially expressed by >1.5-fold in both cell lines after MHT treatment. Of the 1,196 commonly and differentially expressed probe sets analysed by k-means clustering, three gene clusters, Up-I, Down-I and Down-II, were observed. Interestingly, two gene networks were obtained from the up-regulated genes in cluster Up-I. The gene network E contained DDIT3 and HSPA5 and was mainly associated with the biological process of endoplasmic reticulum stress, while the network S contained HBEGF and LIF and was associated with the biological process of cell survival. Eighteen genes were validated by quantitative real-time polymerase chain reaction, consistent with the microarray data, in four kinds of NHF cells. Conclusions: Common genes that were differentially expressed and/or acted within a gene network in response to MHT in NHF cells were identified. These findings provide the molecular basis for a further understanding of the mechanisms of the MHT response in NHF cells.

AB - Purpose: Heat stress induces complex cellular responses, and its detailed molecular mechanisms still remain to be clarified. The objective of this study was to investigate the molecular mechanisms underlying cellular responses to mild hyperthermia (MHT) in normal human fibroblastic (NHF) cells. Materials and methods: Cells were treated with MHT (41°C, 30min) and then cultured at 37°C. Gene expression was determined by the GeneChip® system and bioinformatics tools. Results: Treatment of the NHF cell lines, Hs68 and OUMS-36, with MHT did not affect the cell viability or cell cycle. In contrast, many probe sets were differentially expressed by >1.5-fold in both cell lines after MHT treatment. Of the 1,196 commonly and differentially expressed probe sets analysed by k-means clustering, three gene clusters, Up-I, Down-I and Down-II, were observed. Interestingly, two gene networks were obtained from the up-regulated genes in cluster Up-I. The gene network E contained DDIT3 and HSPA5 and was mainly associated with the biological process of endoplasmic reticulum stress, while the network S contained HBEGF and LIF and was associated with the biological process of cell survival. Eighteen genes were validated by quantitative real-time polymerase chain reaction, consistent with the microarray data, in four kinds of NHF cells. Conclusions: Common genes that were differentially expressed and/or acted within a gene network in response to MHT in NHF cells were identified. These findings provide the molecular basis for a further understanding of the mechanisms of the MHT response in NHF cells.

KW - gene function

KW - gene network

KW - microarray

KW - Mild temperature hyperthermia

KW - normal human fibroblastic cell

UR - http://www.scopus.com/inward/record.url?scp=84872298409&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872298409&partnerID=8YFLogxK

U2 - 10.3109/02656736.2012.753163

DO - 10.3109/02656736.2012.753163

M3 - Article

C2 - 23311377

AN - SCOPUS:84872298409

VL - 29

SP - 38

EP - 50

JO - International Journal of Hyperthermia

JF - International Journal of Hyperthermia

SN - 0265-6736

IS - 1

ER -