Common marmoset embryonic stem cell can differentiate into cardiomyocytes

Hao Chen, Fumiyuki Hattori, Mitsushige Murata, Weizhen Li, Shinsuke Yuasa, Takeshi Onizuka, Kenichiro Shimoji, Yohei Ohno, Erika Sasaki, Kensuke Kimura, Daihiko Hakuno, Motoaki Sano, Shinji Makino, Satoshi Ogawa, Keiichi Fukuda

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Common marmoset monkeys have recently attracted much attention as a primate research model, and are preferred to rhesus and cynomolgus monkeys due to their small bodies, easy handling and efficient breeding. We recently reported the establishment of common marmoset embryonic stem cell (CMESC) lines that could differentiate into three germ layers. Here, we report that our CMESC can also differentiate into cardiomyocytes and investigated their characteristics. After induction, FOG-2 was expressed, followed by GATA4 and Tbx20, then Nkx2.5 and Tbx5. Spontaneous beating could be detected at days 12-15. Immunofluorescent staining and ultrastructural analyses revealed that they possessed characteristics typical of functional cardiomyocytes. They showed sinus node-like action potentials, and the beating rate was augmented by isoproterenol stimulation. The BrdU incorporation assay revealed that CMESC-derived cardiomyocytes retained a high proliferative potential for up to 24 weeks. We believe that CMESC-derived cardiomyocytes will advance preclinical studies in cardiovascular regenerative medicine.

Original languageEnglish
Pages (from-to)801-806
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number3
DOIs
Publication statusPublished - 2008 May 9

Keywords

  • Cardiomyocytes
  • Characterization
  • Common marmoset
  • Differentiation
  • Embryonic stem cell
  • Heart regeneration
  • Monkey
  • Preclinical model
  • Primate

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Chen, H., Hattori, F., Murata, M., Li, W., Yuasa, S., Onizuka, T., Shimoji, K., Ohno, Y., Sasaki, E., Kimura, K., Hakuno, D., Sano, M., Makino, S., Ogawa, S., & Fukuda, K. (2008). Common marmoset embryonic stem cell can differentiate into cardiomyocytes. Biochemical and Biophysical Research Communications, 369(3), 801-806. https://doi.org/10.1016/j.bbrc.2008.02.141