Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies

Olga Amengual, Tetsuya Horita, Walter Binder, Gary L. Norman, Zakera Shums, Masaru Kato, Kotaro Otomo, Yuichiro Fujieda, Kenji Oku, Toshiyuki Bohgaki, Shinsuke Yasuda, Tatsuya Atsumi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Phosphatidylserine-dependent antiprothrombin antibodies (aPS/PT) were strongly correlated with the presence of lupus anticoagulant showing a high specificity for the diagnosis of antiphospholipid syndrome. However, the main criticism for the clinical applicability of aPS/PT testing is the lack of reproducibility of the results among laboratories. In this study, we measured IgG and IgM aPS/PT using our original in-house enzyme-linked immunosorbent assays (ELISA) and commercial ELISA kits to assess the assay performance and to evaluate the accuracy of aPS/PT results. The study included 111 plasma samples collected from patients and stored at our laboratory for aPS/PT assessment. Sixty-one samples were tested for IgG aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite™ aPS/PT IgG ELISA kit (INOVA Diagnostics, Inc., USA). Fifty samples were evaluated for IgM aPS/PT using two assays: (1) aPS/PT in-house ELISA and (2) QUANTA Lite™ aPS/PT IgM ELISA kit (INOVA Diagnostics). Ninety-eight percent of samples yielded concordant results for IgG aPS/PT and 82 % for IgM aPS/PT. There was an excellent agreement between the IgG aPS/PT assays (Cohen κ = 0.962) and moderate agreement between the IgM aPS/PT assays (κ = 0.597). Statistically significant correlations in the aPS/PT results were obtained from both IgG and IgM aPS/PT assays (r = 0.749, r = 0.622, p < 0.001, respectively). In conclusion, IgG and IgM detection by ELISA is accurate. The performance of aPS/PT is reliable, and concordant results can be obtained using different ELISA methods.

Original languageEnglish
Pages (from-to)1225-1230
Number of pages6
JournalRheumatology International
Volume34
Issue number9
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Phosphatidylserines
Immunoenzyme Techniques
Immunoglobulin M
Enzyme-Linked Immunosorbent Assay
Immunoglobulin G
Antibodies
Lupus Coagulation Inhibitor
Antiphospholipid Syndrome
Reproducibility of Results

Keywords

  • Antiphospholipid antibodies
  • Antiphospholipid syndrome
  • Lupus anticoagulant
  • Thrombosis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies. / Amengual, Olga; Horita, Tetsuya; Binder, Walter; Norman, Gary L.; Shums, Zakera; Kato, Masaru; Otomo, Kotaro; Fujieda, Yuichiro; Oku, Kenji; Bohgaki, Toshiyuki; Yasuda, Shinsuke; Atsumi, Tatsuya.

In: Rheumatology International, Vol. 34, No. 9, 01.01.2014, p. 1225-1230.

Research output: Contribution to journalArticle

Amengual, O, Horita, T, Binder, W, Norman, GL, Shums, Z, Kato, M, Otomo, K, Fujieda, Y, Oku, K, Bohgaki, T, Yasuda, S & Atsumi, T 2014, 'Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies', Rheumatology International, vol. 34, no. 9, pp. 1225-1230. https://doi.org/10.1007/s00296-014-2951-0
Amengual, Olga ; Horita, Tetsuya ; Binder, Walter ; Norman, Gary L. ; Shums, Zakera ; Kato, Masaru ; Otomo, Kotaro ; Fujieda, Yuichiro ; Oku, Kenji ; Bohgaki, Toshiyuki ; Yasuda, Shinsuke ; Atsumi, Tatsuya. / Comparative analysis of different enzyme immunoassays for assessment of phosphatidylserine-dependent antiprothrombin antibodies. In: Rheumatology International. 2014 ; Vol. 34, No. 9. pp. 1225-1230.
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