Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues

Junko Ohki, Akihiko Sakashita, Eitaro Aihara, Akihiko Inaba, Hironobu Uchiyama, Masahito Matsumoto, Yuzo Ninomiya, Takumi Yamane, Yuichi Oishi, Ken Iwatsuki

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.

Original languageEnglish
Pages (from-to)936-942
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume84
Issue number5
DOIs
Publication statusPublished - 2020 May 3

Keywords

  • Ngn3
  • Organoid
  • hormone
  • intestine

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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