TY - JOUR
T1 - Comparative analysis of enteroendocrine cells and their hormones between mouse intestinal organoids and native tissues
AU - Ohki, Junko
AU - Sakashita, Akihiko
AU - Aihara, Eitaro
AU - Inaba, Akihiko
AU - Uchiyama, Hironobu
AU - Matsumoto, Masahito
AU - Ninomiya, Yuzo
AU - Yamane, Takumi
AU - Oishi, Yuichi
AU - Iwatsuki, Ken
N1 - Funding Information:
This work was supported by the MEXT-Supported Program for the Strategic Research Foundation at Private Universities 2013?2017 (S1311017), JSPS KAKENHI (208920 to K.I., 5H02571 to Y.N.), The Japan Chemical Research Foundation (to K.I.), Grant-in-Aid for Advanced Research Project (to K.I.) and Grant for Ph.D. Research (to J.O.) from Tokyo University of Agriculture. We thank Dr. Clevers for Wnt3a and Noggin-producing cell lines and Dr. Jeffery Whitsett for the R-spondin-producing cell line. We also thank the Genome Research Center at Tokyo University of Agriculture for performing RNA-Seq and bioinformatic analyses.
PY - 2020/5/3
Y1 - 2020/5/3
N2 - Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.
AB - Endocrine cells in the gastrointestinal tract secrete multiple hormones to maintain homeostasis in the body. In the present study, we generated intestinal organoids from the duodenum, jejunum, and ileum of Neurogenin 3 (Ngn3)-EGFP mice and examined how enteroendocrine cells (EECs) within organoid cultures resemble native epithelial cells in the gut. Transcriptome analysis of EGFP-positive cells from Ngn3-EGFP organoids showed gene expression pattern comparable to EECs in vivo. We also compared mRNAs of five major hormones, namely, ghrelin (Ghrl), cholecystokinin (Cck), Gip, secretin (Sct), and glucagon (Gcg) in organoids and small intestine along the longitudinal axis and found that expression patterns of these hormones in organoids were similar to those in native tissues. These findings suggest that an intestinal organoid culture system can be utilized as a suitable model to study enteroendocrine cell functions in vitro.
KW - Ngn3
KW - Organoid
KW - hormone
KW - intestine
UR - http://www.scopus.com/inward/record.url?scp=85077880550&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85077880550&partnerID=8YFLogxK
U2 - 10.1080/09168451.2020.1713043
DO - 10.1080/09168451.2020.1713043
M3 - Article
C2 - 31916916
AN - SCOPUS:85077880550
VL - 84
SP - 936
EP - 942
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 5
ER -