Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species

Maud Vallée, Kazuhiro Aiba, Yulan Piao, Marie France Palin, Minoru Ko, Marc André Sirard

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Cross-species comparison of gene expression is a powerful approach for discovering genes that have been conserved throughout evolution. Conserved genes are presumably very important in the mechanisms related to the unique molecular functions in oocytes. The objective of this study was to identify genes expressed in the oocyte and conserved across three diverse vertebrate species. We report the global gene expression profiles of Bos taurus and Xenopus laevis oocytes on an NIA mouse development microarray that consists of 60-mer oligonucleotide probes representing more than 20 000 mouse transcripts derived from stem cell, oocyte, and early embryo cDNA libraries. Analysis based on intensity values revealed that 9853 and 10 046 genes are expressed in bovine and Xenopus oocytes respectively. Furthermore, previously published microarray data on preimplantation development in the mouse were used for a comparative analysis of global oocyte gene expression profiles. Interestingly, a substantial proportion of the genes expressed in mouse oocytes is conserved between the three species (74%, 7275 genes). Moreover, functional annotation of these conserved oocyte-expressed genes confirmed that certain functions are conserved among the three species. RNA metabolism and cell cycle were among the over-represented Gene Ontology terms in the biological process category. Finally, a pattern-matching analysis identified 208 conserved maternally expressed genes. Results from these cross-species hybridizations allowed numerous genes expressed in oocytes and conserved between Mus musculus, B. taurus, and X. laevis to be identified. This comparative analysis of oocyte transcript profiles revealed a high degree of conservation among species.

Original languageEnglish
Pages (from-to)439-448
Number of pages10
JournalReproduction
Volume135
Issue number4
DOIs
Publication statusPublished - 2008 Apr
Externally publishedYes

Fingerprint

Oocytes
Genes
Xenopus laevis
Transcriptome
Biological Phenomena
Gene Ontology
Oligonucleotide Probes
Xenopus
Gene Library
Vertebrates
Cell Cycle
Stem Cells
Embryonic Structures
RNA
Gene Expression

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species. / Vallée, Maud; Aiba, Kazuhiro; Piao, Yulan; Palin, Marie France; Ko, Minoru; Sirard, Marc André.

In: Reproduction, Vol. 135, No. 4, 04.2008, p. 439-448.

Research output: Contribution to journalArticle

Vallée, Maud ; Aiba, Kazuhiro ; Piao, Yulan ; Palin, Marie France ; Ko, Minoru ; Sirard, Marc André. / Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species. In: Reproduction. 2008 ; Vol. 135, No. 4. pp. 439-448.
@article{aee37e8729ed464c936109ec8dfcdb5c,
title = "Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species",
abstract = "Cross-species comparison of gene expression is a powerful approach for discovering genes that have been conserved throughout evolution. Conserved genes are presumably very important in the mechanisms related to the unique molecular functions in oocytes. The objective of this study was to identify genes expressed in the oocyte and conserved across three diverse vertebrate species. We report the global gene expression profiles of Bos taurus and Xenopus laevis oocytes on an NIA mouse development microarray that consists of 60-mer oligonucleotide probes representing more than 20 000 mouse transcripts derived from stem cell, oocyte, and early embryo cDNA libraries. Analysis based on intensity values revealed that 9853 and 10 046 genes are expressed in bovine and Xenopus oocytes respectively. Furthermore, previously published microarray data on preimplantation development in the mouse were used for a comparative analysis of global oocyte gene expression profiles. Interestingly, a substantial proportion of the genes expressed in mouse oocytes is conserved between the three species (74{\%}, 7275 genes). Moreover, functional annotation of these conserved oocyte-expressed genes confirmed that certain functions are conserved among the three species. RNA metabolism and cell cycle were among the over-represented Gene Ontology terms in the biological process category. Finally, a pattern-matching analysis identified 208 conserved maternally expressed genes. Results from these cross-species hybridizations allowed numerous genes expressed in oocytes and conserved between Mus musculus, B. taurus, and X. laevis to be identified. This comparative analysis of oocyte transcript profiles revealed a high degree of conservation among species.",
author = "Maud Vall{\'e}e and Kazuhiro Aiba and Yulan Piao and Palin, {Marie France} and Minoru Ko and Sirard, {Marc Andr{\'e}}",
year = "2008",
month = "4",
doi = "10.1530/REP-07-0342",
language = "English",
volume = "135",
pages = "439--448",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "4",

}

TY - JOUR

T1 - Comparative analysis of oocyte transcript profiles reveals a high degree of conservation among species

AU - Vallée, Maud

AU - Aiba, Kazuhiro

AU - Piao, Yulan

AU - Palin, Marie France

AU - Ko, Minoru

AU - Sirard, Marc André

PY - 2008/4

Y1 - 2008/4

N2 - Cross-species comparison of gene expression is a powerful approach for discovering genes that have been conserved throughout evolution. Conserved genes are presumably very important in the mechanisms related to the unique molecular functions in oocytes. The objective of this study was to identify genes expressed in the oocyte and conserved across three diverse vertebrate species. We report the global gene expression profiles of Bos taurus and Xenopus laevis oocytes on an NIA mouse development microarray that consists of 60-mer oligonucleotide probes representing more than 20 000 mouse transcripts derived from stem cell, oocyte, and early embryo cDNA libraries. Analysis based on intensity values revealed that 9853 and 10 046 genes are expressed in bovine and Xenopus oocytes respectively. Furthermore, previously published microarray data on preimplantation development in the mouse were used for a comparative analysis of global oocyte gene expression profiles. Interestingly, a substantial proportion of the genes expressed in mouse oocytes is conserved between the three species (74%, 7275 genes). Moreover, functional annotation of these conserved oocyte-expressed genes confirmed that certain functions are conserved among the three species. RNA metabolism and cell cycle were among the over-represented Gene Ontology terms in the biological process category. Finally, a pattern-matching analysis identified 208 conserved maternally expressed genes. Results from these cross-species hybridizations allowed numerous genes expressed in oocytes and conserved between Mus musculus, B. taurus, and X. laevis to be identified. This comparative analysis of oocyte transcript profiles revealed a high degree of conservation among species.

AB - Cross-species comparison of gene expression is a powerful approach for discovering genes that have been conserved throughout evolution. Conserved genes are presumably very important in the mechanisms related to the unique molecular functions in oocytes. The objective of this study was to identify genes expressed in the oocyte and conserved across three diverse vertebrate species. We report the global gene expression profiles of Bos taurus and Xenopus laevis oocytes on an NIA mouse development microarray that consists of 60-mer oligonucleotide probes representing more than 20 000 mouse transcripts derived from stem cell, oocyte, and early embryo cDNA libraries. Analysis based on intensity values revealed that 9853 and 10 046 genes are expressed in bovine and Xenopus oocytes respectively. Furthermore, previously published microarray data on preimplantation development in the mouse were used for a comparative analysis of global oocyte gene expression profiles. Interestingly, a substantial proportion of the genes expressed in mouse oocytes is conserved between the three species (74%, 7275 genes). Moreover, functional annotation of these conserved oocyte-expressed genes confirmed that certain functions are conserved among the three species. RNA metabolism and cell cycle were among the over-represented Gene Ontology terms in the biological process category. Finally, a pattern-matching analysis identified 208 conserved maternally expressed genes. Results from these cross-species hybridizations allowed numerous genes expressed in oocytes and conserved between Mus musculus, B. taurus, and X. laevis to be identified. This comparative analysis of oocyte transcript profiles revealed a high degree of conservation among species.

UR - http://www.scopus.com/inward/record.url?scp=42549154322&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42549154322&partnerID=8YFLogxK

U2 - 10.1530/REP-07-0342

DO - 10.1530/REP-07-0342

M3 - Article

VL - 135

SP - 439

EP - 448

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 4

ER -