Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes

Dongru Qiu, Kyoko Fujita, Yuko Sakuma, Teruo Tanaka, Yoshiaki Ohashi, Hideyuki Ohshima, Masaru Tomita, Mitsuhiro Itaya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The complete SfiI and I-CeuI physical maps of four Bacillus subtilis (natto) strains, which were previously isolated as natto (fermented soybean) starters, were constructed to elucidate the genome structure. Not only the similarity in genome size and organization but also the microheterogeneity of the gene context was revealed. No large-scale genome rearrangements among the four strains were indicated by mapping of the genes, including 10 rRNA operons (rrn) and relevant genes required for natto production, to the loci corresponding to those of the B. subtilis strain Marburg 168. However, restriction fragment length polymorphism and the presence or absence of strain-specific DNA sequences, such as the prophages SPβ, skin element, and PBSX, as well as the insertion element IS4Bsu1, could be used to identify one of these strains as a Marburg type and the other three strains as natto types. The genome structure and gene heterogeneity were also consistent with the type of indigenous plasmids harbored by the strains.

Original languageEnglish
Pages (from-to)6247-6256
Number of pages10
JournalApplied and Environmental Microbiology
Volume70
Issue number10
DOIs
Publication statusPublished - 2004 Oct

Fingerprint

natto
Soy Foods
physical chromosome mapping
Bacillus subtilis
genome
Genome
gene
rRNA Operon
Genes
Prophages
Genome Size
Chromosome Mapping
Soybeans
Restriction Fragment Length Polymorphisms
Plasmids
plasmid
soybean
skin
Skin
polymorphism

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Qiu, D., Fujita, K., Sakuma, Y., Tanaka, T., Ohashi, Y., Ohshima, H., ... Itaya, M. (2004). Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes. Applied and Environmental Microbiology, 70(10), 6247-6256. https://doi.org/10.1128/AEM.70.10.6247-6256.2004

Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes. / Qiu, Dongru; Fujita, Kyoko; Sakuma, Yuko; Tanaka, Teruo; Ohashi, Yoshiaki; Ohshima, Hideyuki; Tomita, Masaru; Itaya, Mitsuhiro.

In: Applied and Environmental Microbiology, Vol. 70, No. 10, 10.2004, p. 6247-6256.

Research output: Contribution to journalArticle

Qiu, D, Fujita, K, Sakuma, Y, Tanaka, T, Ohashi, Y, Ohshima, H, Tomita, M & Itaya, M 2004, 'Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes', Applied and Environmental Microbiology, vol. 70, no. 10, pp. 6247-6256. https://doi.org/10.1128/AEM.70.10.6247-6256.2004
Qiu, Dongru ; Fujita, Kyoko ; Sakuma, Yuko ; Tanaka, Teruo ; Ohashi, Yoshiaki ; Ohshima, Hideyuki ; Tomita, Masaru ; Itaya, Mitsuhiro. / Comparative analysis of physical maps of four Bacillus subtilis (natto) genomes. In: Applied and Environmental Microbiology. 2004 ; Vol. 70, No. 10. pp. 6247-6256.
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