TY - JOUR
T1 - Comparative analysis of the expression patterns of UPR-target genes caused by upr-inducing compounds
AU - Shinjo, Satoko
AU - Mizotani, Yuji
AU - Tashiro, Etsu
AU - Imoto, Masaya
N1 - Funding Information:
This work is supported by Grants-in-Aid for Scientific Research (KAKENHI grant no. 23510283, to E.T.) from Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. S.S. was a research assistant in the Global COE Program for Human Metabolomic Systems Biology.
PY - 2013
Y1 - 2013
N2 - Endoplasmic reticulum (ER) stress, due to an accumulation of unfolded proteins in the ER, leads to a process known as the unfolded protein response (UPR). Since the several compounds used to induce UPR have different modes of action, their mechanisms of protein accumulation are thought to be different, but it is unclear whether these compounds can upregulate UPR target genes with similar kinetics. Hence, we sought to compare the expression patterns of nine UPR target genes induced by seven UPR-inducing compounds. Hierarchical clustering analysis revealed that the expression patterns of the UPR target genes induced by the seven compounds were classified into two clusters; cluster A (thapsigargin, tunicamycin, 2-deoxyglucose, and dithiothreitol) and cluster B (brefeldin A, monensin, and eeyarestatin I). Thus, this study suggests the existence of at least two types of UPR target gene expression profiles, which depend on the mode of action of the compounds.
AB - Endoplasmic reticulum (ER) stress, due to an accumulation of unfolded proteins in the ER, leads to a process known as the unfolded protein response (UPR). Since the several compounds used to induce UPR have different modes of action, their mechanisms of protein accumulation are thought to be different, but it is unclear whether these compounds can upregulate UPR target genes with similar kinetics. Hence, we sought to compare the expression patterns of nine UPR target genes induced by seven UPR-inducing compounds. Hierarchical clustering analysis revealed that the expression patterns of the UPR target genes induced by the seven compounds were classified into two clusters; cluster A (thapsigargin, tunicamycin, 2-deoxyglucose, and dithiothreitol) and cluster B (brefeldin A, monensin, and eeyarestatin I). Thus, this study suggests the existence of at least two types of UPR target gene expression profiles, which depend on the mode of action of the compounds.
KW - Endoplasmic reticulum (ER) stress
KW - Profiling
KW - UPR-inducing compound
KW - Unfolded protein response (UPR)
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U2 - 10.1271/bbb.120812
DO - 10.1271/bbb.120812
M3 - Article
C2 - 23563539
AN - SCOPUS:84878784750
SN - 0916-8451
VL - 77
SP - 729
EP - 735
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 4
ER -