Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system

S. Mahattanadul, Tomonori Nakamura, P. Panichayupakaranant, N. Phdoongsombut, K. Tungsinmunkong, P. Bouking

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The antiulcer effect of bisdemethoxycurcumin, a yellow pigment found mainly in rhizomes of Curcuma longa, was compared with curcumin in gastric ulcer model systems to validate its clinical application as a remedy for peptic ulcer. Western blot analysis of mouse macrophage cell line RAW 264.7 activated with lipopolysaccharide showed that bisdemethoxycurcumin inhibited inducible nitric oxide synthase (iNOS) production significantly but had no effect on tumor necrosis factor-alpha (TNF-α) production, whereas curcumin showed stronger suppression of iNOS protein production and inhibited TNF-α protein production significantly. However, bisdemethoxycurcumin and curcumin possessed similar potency in scavenging nitric oxide generated from mouse macrophage cell line RAW 264.7. Reverse-transcriptase polymerase chain reaction (RT-PCR) analysis showed that both curcuminoids inhibited the induction of iNOS dose-dependently at the transcriptional level and curcumin also appeared to inhibit the induction of TNF-α at post-transcriptional level. In an animal model, intraduodenal administration of bisdemethoxycurcumin (5-80 mg/kg body wt.) showed a strong inhibitory effect on gastric acid secretion in pylorus-ligated rats whereas curcumin (5-20 mg/kg body wt.) showed a less inhibitory effect, with maximum potency at a dose of 20 mg/kg body wt. Moreover, oral administration of bisdemethoxycurcumin at doses of 20-80 mg/kg body wt. twice daily for 10 days showed a significant curative efficacy in accelerating the healing of acetic acid-induced chronic gastric ulcer and promotion of mucosal regeneration in the ulcerated portion in a dose-related manner with potency equal to curcumin. In contrast, the curative potency of curcumin tended to decrease at doses over 160 mg/kg body wt./day. Western blot analysis in ulcerated gastric mucosa showed that bisdemethoxycurcumin dose-dependently reduced the increased protein expression level of iNOS but not TNF-α. These results indicated that bisdemethoxycurcumin directly accelerates gastric ulcer healing with potency equal to curcumin. Its antiulcer effect might be due to its properties of decreasing gastric acid secretion and enhancing the mucosal defensive mechanism through suppression of iNOS-mediated inflammation.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalPhytomedicine
Volume16
Issue number4
DOIs
Publication statusPublished - 2009 Apr
Externally publishedYes

Fingerprint

Curcumin
Stomach Ulcer
Nitric Oxide Synthase Type II
Tumor Necrosis Factor-alpha
Gastric Acid
Western Blotting
Macrophages
Curcuma
Rhizome
Proteins
bis(4-hydroxycinnamoyl)methane
Pylorus
Gastric Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Peptic Ulcer
Acetic Acid
Oral Administration
Lipopolysaccharides
Regeneration
Nitric Oxide

Keywords

  • Bisdemethoxycurcumin
  • Curcumin
  • Gastric ulcer
  • Inducible nitric oxide synthase (iNOS)
  • Inflammatory cytokines
  • Tumor necrosis factor-alpha (TNF-α)

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Mahattanadul, S., Nakamura, T., Panichayupakaranant, P., Phdoongsombut, N., Tungsinmunkong, K., & Bouking, P. (2009). Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system. Phytomedicine, 16(4), 342-351. https://doi.org/10.1016/j.phymed.2008.12.005

Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system. / Mahattanadul, S.; Nakamura, Tomonori; Panichayupakaranant, P.; Phdoongsombut, N.; Tungsinmunkong, K.; Bouking, P.

In: Phytomedicine, Vol. 16, No. 4, 04.2009, p. 342-351.

Research output: Contribution to journalArticle

Mahattanadul, S, Nakamura, T, Panichayupakaranant, P, Phdoongsombut, N, Tungsinmunkong, K & Bouking, P 2009, 'Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system', Phytomedicine, vol. 16, no. 4, pp. 342-351. https://doi.org/10.1016/j.phymed.2008.12.005
Mahattanadul S, Nakamura T, Panichayupakaranant P, Phdoongsombut N, Tungsinmunkong K, Bouking P. Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system. Phytomedicine. 2009 Apr;16(4):342-351. https://doi.org/10.1016/j.phymed.2008.12.005
Mahattanadul, S. ; Nakamura, Tomonori ; Panichayupakaranant, P. ; Phdoongsombut, N. ; Tungsinmunkong, K. ; Bouking, P. / Comparative antiulcer effect of Bisdemethoxycurcumin and Curcumin in a gastric ulcer model system. In: Phytomedicine. 2009 ; Vol. 16, No. 4. pp. 342-351.
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