Comparative Metabolomics of Small Molecules Specifically Expressed in the Dorsal or Ventral Marginal Zones in Vertebrate Gastrula

Yukako Suzuki, Ryosuke Hayasaka, Masako Hasebe, Satsuki Ikeda, Tomoyoshi Soga, Masaru Tomita, Akiyoshi Hirayama, Hiroki Kuroda

Research output: Contribution to journalArticlepeer-review

Abstract

Many previous studies have reported the various proteins specifically secreted as inducers in the dorsal or ventral regions in vertebrate gastrula. However, little is known about the effect on cell fate of small molecules below 1000 Da. We therefore tried to identify small molecules specifically expressed in the dorsal marginal zone (DMZ) or ventral marginal zone (VMZ) in vertebrate gastrula. Small intracellular and secreted molecules were detected using explants and supernatant samples. Hydrophilic metabolites were analyzed by capillary ion chromatography–mass spectrometry and liquid chromatography–mass spectrometry, and lipids were analyzed by supercritical fluid chromatography–tandem mass spectrometry. In total, 190 hydrophilic metabolites and 396 lipids were identified. The DMZ was found to have high amounts of glycolysis-and glutathione metabolismrelated metabolites in explants, and the VMZ was richer in purine metabolism-related metabolites. We also discovered some hydrophilic metabolites and lipids differentially contained in the DMZ or VMZ. Our research would contribute to a deeper understanding of the cellular physiology that regulates early embryogenesis.

Original languageEnglish
Article number566
JournalMetabolites
Volume12
Issue number6
DOIs
Publication statusPublished - 2022 Jun

Keywords

  • dorsal–ventral patterning
  • early embryogenesis
  • metabolomic analysis
  • Spemann organizer
  • Xenopus laevis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

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