Comparative study of eye defective worm 'menashi' and regenerating wild-type in planarian, Dugesia ryukyuensis

Yukiko Sato, Kazuya Kobayashi, Midori Matsumoto, Motonori Hoshi, Sumiko Negishi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Inbreeding of the sexualized planarian, Dugesia ryukyuensis, produces eye-defective worms, menashi, in the F1 population. To study the effects of this mutation on the eye, we observed the eye-region of menashi using electron microscopy and compared it with the regenerating eye in wild-type worms. The intact eye of wild-type planarians consisted of a few pigment cells and a number of visual cells. Pigment cells containing spherically-shaped electron-dense melanosomes contacted each other and enclosed rhabdomes of visual cells. Rhabdomes had numerous tubular microvilli extending radially and touching the pigment cells. However, in menashi, various lengths of tubular microvilli were irregularly distributed near the pigment cells, which contained numerous electron-lucent premelanosomes, and no adhesive structures were found between the pigment cells. The premelanosomes of menashi were equal in size to those seen after 2 days of regeneration in wild-type planarians and were similar in maturation to those found after 3 days of regeneration in wild-type planarian. These results suggest that menashi is defective in the mechanism(s) of developing pigment granules and constructing visual cells. These findings also suggest that pigment cells in menashi are defective in the mechanism(s) involved with cell adhesion.

Original languageEnglish
Pages (from-to)86-91
Number of pages6
JournalPigment Cell Research
Volume18
Issue number2
DOIs
Publication statusPublished - 2005 Apr

Fingerprint

Planarians
Dugesia
Pigments
eyes
pigments
cells
Microvilli
microvilli
Regeneration
Electrons
Melanosomes
electrons
Cell adhesion
Inbreeding
Electron microscopy
Cell Adhesion
Adhesives
cell adhesion
adhesives
Electron Microscopy

Keywords

  • Eye-defective
  • Ocellus
  • Pigment cell
  • Planarian
  • Regeneration

ASJC Scopus subject areas

  • Cell Biology
  • Agronomy and Crop Science
  • Plant Science
  • Clinical Biochemistry
  • Developmental Biology

Cite this

Comparative study of eye defective worm 'menashi' and regenerating wild-type in planarian, Dugesia ryukyuensis. / Sato, Yukiko; Kobayashi, Kazuya; Matsumoto, Midori; Hoshi, Motonori; Negishi, Sumiko.

In: Pigment Cell Research, Vol. 18, No. 2, 04.2005, p. 86-91.

Research output: Contribution to journalArticle

Sato, Yukiko ; Kobayashi, Kazuya ; Matsumoto, Midori ; Hoshi, Motonori ; Negishi, Sumiko. / Comparative study of eye defective worm 'menashi' and regenerating wild-type in planarian, Dugesia ryukyuensis. In: Pigment Cell Research. 2005 ; Vol. 18, No. 2. pp. 86-91.
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