Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity

Fernando Ulloa-Montoya, Benjamin L. Kidder, Karen A. Pauwelyn, Lucas G. Chase, Aernout Luttun, Annelies Crabbe, Martine Geraerts, Alexei A. Sharov, Yulan Piao, Minoru Ko, Wei Shou Hu, Catherine M. Verfaillie

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111 Citations (Scopus)

Abstract

Background: Recently, several populations of postnatal stem cells, such as multipotent adult progenitor cells (MAPCs), have been described that have broader differentiation ability than classical adult stem cells. Here we compare the transcriptome of pluripotent embryonic stem cells (ESCs), MAPCs, and lineage-restricted mesenchymal stem cells (MSCs) to determine their relationship. Results: Applying principal component analysis, non-negative matrix factorization and k-means clustering algorithms to the gene-expression data, we identified a unique gene-expression profile for MAPCs. Apart from the ESC-specific transcription factor Oct4 and other ESC transcripts, some of them associated with maintaining ESC pluripotency, MAPCs also express transcripts characteristic of early endoderm and mesoderm. MAPCs do not, however, express Nanog or Sox2, two other key transcription factors involved in maintaining ESC properties. This unique molecular signature was seen irrespective of the microarray platform used and was very similar for both mouse and rat MAPCs. As MSC-like cells isolated under MAPC conditions are virtually identical to MSCs, and MSCs cultured in MAPC conditions do not upregulate MAPC-expressed transcripts, the MAPC signature is cell-type specific and not merely the result of differing culture conditions. Conclusion: Multivariate analysis techniques clustered stem cells on the basis of their expressed gene profile, and the genes determining this clustering reflected the stem cells' differentiation potential in vitro. This comparative transcriptome analysis should significantly aid the isolation and culture of MAPCs and MAPC-like cells, and form the basis for studies to gain insights into genes that confer on these cells their greater developmental potency.

Original languageEnglish
Article numberR163
JournalGenome Biology
Volume8
Issue number8
DOIs
Publication statusPublished - 2007
Externally publishedYes

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Adult Stem Cells
embryonic stem cells
Gene Expression Profiling
Embryonic Stem Cells
transcriptomics
stem cells
Stem Cells
stem
Mesenchymal Stromal Cells
adult stem cells
analysis
gene expression
gene
Transcriptome
Cluster Analysis
Transcription Factors
Genes
Pluripotent Stem Cells
Endoderm
transcription factors

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Ulloa-Montoya, F., Kidder, B. L., Pauwelyn, K. A., Chase, L. G., Luttun, A., Crabbe, A., ... Verfaillie, C. M. (2007). Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity. Genome Biology, 8(8), [R163]. https://doi.org/10.1186/gb-2007-8-8-r163

Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity. / Ulloa-Montoya, Fernando; Kidder, Benjamin L.; Pauwelyn, Karen A.; Chase, Lucas G.; Luttun, Aernout; Crabbe, Annelies; Geraerts, Martine; Sharov, Alexei A.; Piao, Yulan; Ko, Minoru; Hu, Wei Shou; Verfaillie, Catherine M.

In: Genome Biology, Vol. 8, No. 8, R163, 2007.

Research output: Contribution to journalArticle

Ulloa-Montoya, F, Kidder, BL, Pauwelyn, KA, Chase, LG, Luttun, A, Crabbe, A, Geraerts, M, Sharov, AA, Piao, Y, Ko, M, Hu, WS & Verfaillie, CM 2007, 'Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity', Genome Biology, vol. 8, no. 8, R163. https://doi.org/10.1186/gb-2007-8-8-r163
Ulloa-Montoya, Fernando ; Kidder, Benjamin L. ; Pauwelyn, Karen A. ; Chase, Lucas G. ; Luttun, Aernout ; Crabbe, Annelies ; Geraerts, Martine ; Sharov, Alexei A. ; Piao, Yulan ; Ko, Minoru ; Hu, Wei Shou ; Verfaillie, Catherine M. / Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity. In: Genome Biology. 2007 ; Vol. 8, No. 8.
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