Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species

T. Anai, M. Miyata, Seiji Kosemura, S. Yamamura, T. Tsuge, M. Matsui, H. Uchida, K. Hasegawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The cDNA fragments of auxin-binding protein (ABP1) were isolated by degenerated-primer mediated reverse transcription-polymerase chain reaction (RT-PCR) from dicotyledonous plant species, i.e. cress, mung bean, pea, radish and soybean, and monocotyledonous species, i.e. oat and rice. Cloned abp 1 cDNA fragments were sequenced and compared with known abp 1 clones from A. thaliana, maize, strawberry and tobacco at the amino acid level. In all plant species studied, the amino acid sequences of newly isolated abp 1 clones were highly conserved at two regions (region A: Thr-Pro-Ile-His-Arg-His-Ser-Cys-Glu-Glu-Ile/Val-Phe-Ile/Thr/Val-Val-Leu/Pro/V al-Lys-Gly-Xaa-Gly-Thr-Leu/Val; region B: His-Glu-Asp-Leu-Gln-Phe/Val-Leu-Asp/Val-Ile/Val-Ile-Ser-Arg-Pro-Pro), which were previously reported to be important for auxin-binding. On the other hand, some putative residues of amino acids in the region between regions A and B could be found to be specific in dicot and monocot species, respectively. Southern-blot analysis indicated a small abp 1 gene family in all species studied. Northern-blot analysis indicated that the size of abp 1 mRNA transcripts in all species studied was conserved at approximately 850 bp. The phylogenetic tree of ABP 1 was analyzed by the UPGMA method.

Original languageEnglish
Pages (from-to)446-449
Number of pages4
JournalJournal of Plant Physiology
Volume151
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Indoleacetic Acids
Magnoliopsida
lysylglycine
auxins
Complementary DNA
Clone Cells
clones
Brassicaceae
Raphanus
Amino Acids
Fragaria
amino acids
Peas
radishes
Southern Blotting
mung beans
Liliopsida
Soybeans
Northern blotting
Northern Blotting

Keywords

  • ABP1
  • Auxin
  • Auxin-binding protein
  • RT-PCR

ASJC Scopus subject areas

  • Plant Science

Cite this

Anai, T., Miyata, M., Kosemura, S., Yamamura, S., Tsuge, T., Matsui, M., ... Hasegawa, K. (1997). Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species. Journal of Plant Physiology, 151(4), 446-449.

Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species. / Anai, T.; Miyata, M.; Kosemura, Seiji; Yamamura, S.; Tsuge, T.; Matsui, M.; Uchida, H.; Hasegawa, K.

In: Journal of Plant Physiology, Vol. 151, No. 4, 1997, p. 446-449.

Research output: Contribution to journalArticle

Anai, T, Miyata, M, Kosemura, S, Yamamura, S, Tsuge, T, Matsui, M, Uchida, H & Hasegawa, K 1997, 'Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species', Journal of Plant Physiology, vol. 151, no. 4, pp. 446-449.
Anai, T. ; Miyata, M. ; Kosemura, Seiji ; Yamamura, S. ; Tsuge, T. ; Matsui, M. ; Uchida, H. ; Hasegawa, K. / Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species. In: Journal of Plant Physiology. 1997 ; Vol. 151, No. 4. pp. 446-449.
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