TY - JOUR
T1 - Comparison of abp 1 primary sequences from monocotyledonous and dicotyledonous species
AU - Anai, T.
AU - Miyata, M.
AU - Kosemura, S.
AU - Yamamura, S.
AU - Tsuge, T.
AU - Matsui, M.
AU - Uchida, H.
AU - Hasegawa, K.
N1 - Funding Information:
The authors thank Drs. K. Yokotani-Tomita and K. Yamada for hdpful discussion. We are also grateful to N. Kono and R. Takai for technical assistance. This work was supported in part by a Grant-in Aid for Scientific Research on Priority AIeas (No. 06240103) from the Ministry of Education, Science and Culture Japan to K. H.
PY - 1997
Y1 - 1997
N2 - The cDNA fragments of auxin-binding protein (ABP1) were isolated by degenerated-primer mediated reverse transcription-polymerase chain reaction (RT-PCR) from dicotyledonous plant species, i.e. cress, mung bean, pea, radish and soybean, and monocotyledonous species, i.e. oat and rice. Cloned abp 1 cDNA fragments were sequenced and compared with known abp 1 clones from A. thaliana, maize, strawberry and tobacco at the amino acid level. In all plant species studied, the amino acid sequences of newly isolated abp 1 clones were highly conserved at two regions (region A: Thr-Pro-Ile-His-Arg-His-Ser-Cys-Glu-Glu-Ile/Val-Phe-Ile/Thr/Val-Val-Leu/Pro/V al-Lys-Gly-Xaa-Gly-Thr-Leu/Val; region B: His-Glu-Asp-Leu-Gln-Phe/Val-Leu-Asp/Val-Ile/Val-Ile-Ser-Arg-Pro-Pro), which were previously reported to be important for auxin-binding. On the other hand, some putative residues of amino acids in the region between regions A and B could be found to be specific in dicot and monocot species, respectively. Southern-blot analysis indicated a small abp 1 gene family in all species studied. Northern-blot analysis indicated that the size of abp 1 mRNA transcripts in all species studied was conserved at approximately 850 bp. The phylogenetic tree of ABP 1 was analyzed by the UPGMA method.
AB - The cDNA fragments of auxin-binding protein (ABP1) were isolated by degenerated-primer mediated reverse transcription-polymerase chain reaction (RT-PCR) from dicotyledonous plant species, i.e. cress, mung bean, pea, radish and soybean, and monocotyledonous species, i.e. oat and rice. Cloned abp 1 cDNA fragments were sequenced and compared with known abp 1 clones from A. thaliana, maize, strawberry and tobacco at the amino acid level. In all plant species studied, the amino acid sequences of newly isolated abp 1 clones were highly conserved at two regions (region A: Thr-Pro-Ile-His-Arg-His-Ser-Cys-Glu-Glu-Ile/Val-Phe-Ile/Thr/Val-Val-Leu/Pro/V al-Lys-Gly-Xaa-Gly-Thr-Leu/Val; region B: His-Glu-Asp-Leu-Gln-Phe/Val-Leu-Asp/Val-Ile/Val-Ile-Ser-Arg-Pro-Pro), which were previously reported to be important for auxin-binding. On the other hand, some putative residues of amino acids in the region between regions A and B could be found to be specific in dicot and monocot species, respectively. Southern-blot analysis indicated a small abp 1 gene family in all species studied. Northern-blot analysis indicated that the size of abp 1 mRNA transcripts in all species studied was conserved at approximately 850 bp. The phylogenetic tree of ABP 1 was analyzed by the UPGMA method.
KW - ABP1
KW - Auxin
KW - Auxin-binding protein
KW - RT-PCR
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U2 - 10.1016/S0176-1617(97)80010-7
DO - 10.1016/S0176-1617(97)80010-7
M3 - Article
AN - SCOPUS:0030666320
SN - 0176-1617
VL - 151
SP - 446
EP - 449
JO - Z. PFLANZENPHYSIOL.
JF - Z. PFLANZENPHYSIOL.
IS - 4
ER -