Comparison of blood-brain barrier permeability in mice and rats using in situ brain perfusion technique

Hideyasu Murakami, Hitomi Takanaga, Hirotami Matsuo, Hisakazu Ohtani, Yasufumi Sawada

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Here we present a method for measuring the permeability coefficient-surface area product (PS) values at the blood-brain barrier in mice, using the in situ brain perfusion technique originally developed for rats by Takasato et al. (Am J Physiol Heart Circ Physiol 247: H484-H493, 1984). Retrograde infusion into the right external carotid artery increased the carotid perfusion pressure in proportion to the perfusion rate. Intravascular volume and cerebral perfusion fluid flow at a perfusion rate of 1.0 ml/min in mice were similar to those in rats. In addition, the contribution of systemic blood to total flow in the hemisphere was small (only 3.2%). These findings indicated that this perfusion rate is suitable for mice. The PS values of more than 20 different compounds were determined in mice by using the in situ brain perfusion technique, and comparisons were made with data from rats. There was a close relationship (1:1) between the PS values in mice and rats, indicating that brain capillary permeabilities are similar in mice and rats.

Original languageEnglish
Pages (from-to)H1022-H1028
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number3 48-3
Publication statusPublished - 2000 Oct 4
Externally publishedYes



  • Carotid perfusion pressure
  • Cerebral perfusion fluid flow
  • Contribution of systemic blood to total flow
  • Permeability coefficient-surface area product
  • Vascular volume

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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