Comparison of cationic myocardial perfusion agents

Characteristics of accumulation in cultured smooth muscle cells

Kayoko Nakamura, Toshikazu Sammiya, Jun Hashimoto, Ryochi Ishibashi, Kazuhiro Matsumoto, Atsushi Kubo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The uptake and washout kinetics of two cationic lipophilic99mTc-labeled myocardial perfusion agents,99mTc-methoxyisobutylisonitrile (99mTc-MIBI) and99mTc-l,2-bis[bis-(2-ethoxyethyl)-phosphino]ethane (99mTc-Tetrofosmin), were studied in cultured smooth muscle cells and compared to the conventional myocardial perfusion agent,201Tl. Both99mTc-MIBI and99mTc-Tetrofosmin had a 4-fold greater uptake than201Tl, and they were washed out of cells through similar kinetics which had slower rates than201T1. Incubation with metabolism inhibitors had a modest influence on the uptake of these two99mTc-labeled agents, although their extent and inhibited sites were slightly different. Ion transport inhibitors did not affect the uptake of99mTc-MIBI, although the99mTc-Tetrofosmin uptake was slightly inhibited when the Ca2+ channel was blocked. Our studies indicate that99mTc-MIBI and99mTc-Tetrofosmin were taken up by smooth muscle cells in similar pharmacokinetic patterns, but their accumulation reflected a different meaning for cell viability.

Original languageEnglish
Pages (from-to)375-381
Number of pages7
JournalAnnals of Nuclear Medicine
Volume10
Issue number4
DOIs
Publication statusPublished - 1996 Dec

Fingerprint

Smooth Muscle Myocytes
Perfusion
Ethane
Ion Transport
Cell Survival
Pharmacokinetics
technetium 99m methoxyisobutylisonitrile
technetium Tc 99m 1,2-bis(bis(2-ethoxyethyl)phosphino)ethane

Keywords

  • T1
  • Tc-MIBI
  • Tc-tetrofosmin
  • smooth muscle cells

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Comparison of cationic myocardial perfusion agents : Characteristics of accumulation in cultured smooth muscle cells. / Nakamura, Kayoko; Sammiya, Toshikazu; Hashimoto, Jun; Ishibashi, Ryochi; Matsumoto, Kazuhiro; Kubo, Atsushi.

In: Annals of Nuclear Medicine, Vol. 10, No. 4, 12.1996, p. 375-381.

Research output: Contribution to journalArticle

Nakamura, Kayoko ; Sammiya, Toshikazu ; Hashimoto, Jun ; Ishibashi, Ryochi ; Matsumoto, Kazuhiro ; Kubo, Atsushi. / Comparison of cationic myocardial perfusion agents : Characteristics of accumulation in cultured smooth muscle cells. In: Annals of Nuclear Medicine. 1996 ; Vol. 10, No. 4. pp. 375-381.
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