Comparison of clonogenic survival data obtained by pre-and post-irradiation methods

Takahiro Oike; Yuka Hirota; Narisa Dewi Maulany Darwis; Atsushi Shibata; Tatsuya Ohno

doi:10.3390/jpm10040171

Comparison of clonogenic survival data obtained by pre-and post-irradiation methods

Takahiro Oike, Yuka Hirota, Narisa Dewi Maulany Darwis, Atsushi Shibata, Tatsuya Ohno

Research output: Contribution to journal › Article › peer-review

Abstract

Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF₂, SF₄, SF₆, SF₈, D₁₀, or D₅₀ (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.

Original language	English
Article number	171
Pages (from-to)	1-6
Number of pages	6
Journal	Journal of Personalized Medicine
Volume	10
Issue number	4
DOIs	https://doi.org/10.3390/jpm10040171
Publication status	Published - 2020 Dec
Externally published	Yes

Keywords

Cancer
Clonogenic assays
Methods
Plating
Radiation
Radiosensitivity

ASJC Scopus subject areas

Medicine (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/jpm10040171

Cite this

@article{813e91da8442455d942e7ec51f222010,

title = "Comparison of clonogenic survival data obtained by pre-and post-irradiation methods",

abstract = "Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF2, SF4, SF6, SF8, D10, or D50 (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.",

keywords = "Cancer, Clonogenic assays, Methods, Plating, Radiation, Radiosensitivity",

author = "Takahiro Oike and Yuka Hirota and Darwis, {Narisa Dewi Maulany} and Atsushi Shibata and Tatsuya Ohno",

note = "Funding Information: Funding: This research was funded by Grants-in-Aid from the Japan Society for the Promotion of Science for KAKENHI [19K17162]. This work was also supported by Gunma University Heavy Ion Medical Center. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = dec,

doi = "10.3390/jpm10040171",

language = "English",

volume = "10",

pages = "1--6",

journal = "Journal of Personalized Medicine",

issn = "2075-4426",

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TY - JOUR

T1 - Comparison of clonogenic survival data obtained by pre-and post-irradiation methods

AU - Oike, Takahiro

AU - Hirota, Yuka

AU - Darwis, Narisa Dewi Maulany

AU - Shibata, Atsushi

AU - Ohno, Tatsuya

N1 - Funding Information: Funding: This research was funded by Grants-in-Aid from the Japan Society for the Promotion of Science for KAKENHI [19K17162]. This work was also supported by Gunma University Heavy Ion Medical Center. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/12

Y1 - 2020/12

N2 - Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF2, SF4, SF6, SF8, D10, or D50 (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.

AB - Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF2, SF4, SF6, SF8, D10, or D50 (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that supports inter-study comparison and integrated analysis of published clonogenic assay data regardless of the plating method used.

KW - Cancer

KW - Clonogenic assays

KW - Methods

KW - Plating

KW - Radiation

KW - Radiosensitivity

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U2 - 10.3390/jpm10040171

DO - 10.3390/jpm10040171

M3 - Article

AN - SCOPUS:85092694694

SN - 2075-4426

VL - 10

SP - 1

EP - 6

JO - Journal of Personalized Medicine

JF - Journal of Personalized Medicine

IS - 4

M1 - 171

ER -

Comparison of clonogenic survival data obtained by pre-and post-irradiation methods

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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