Abstract
We performed multi-b and multi-diffusion-time diffusion-weighted magnetic resonance imaging on aquaporin-4-expressing (AQ) and -non-expressing (noAQ) cells, and demonstrated a clear difference between the signals from the two cell types. The data were interpreted using a two-compartment (intra and extracellular spaces) model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange-times obtained with this model are compared to those measured using an independent optical imaging technique (coherent anti-Stokes Raman scattering imaging, CARS). For both techniques it was found that the exchange-time estimated for the noAQ cells was significantly longer than that for the AQ cells.
| Original language | English |
|---|---|
| Article number | 17954 |
| Journal | Scientific reports |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2018 Dec 1 |
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Cite this
Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells. / Obata, Takayuki; Kershaw, Jeff; Tachibana, Yasuhiko; Miyauchi, Takayuki; Abe, Yoichiro; Shibata, Sayaka; Kawaguchi, Hiroshi; Ikoma, Yoko; Takuwa, Hiroyuki; Aoki, Ichio; Yasui, Masato.
In: Scientific reports, Vol. 8, No. 1, 17954, 01.12.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells
AU - Obata, Takayuki
AU - Kershaw, Jeff
AU - Tachibana, Yasuhiko
AU - Miyauchi, Takayuki
AU - Abe, Yoichiro
AU - Shibata, Sayaka
AU - Kawaguchi, Hiroshi
AU - Ikoma, Yoko
AU - Takuwa, Hiroyuki
AU - Aoki, Ichio
AU - Yasui, Masato
PY - 2018/12/1
Y1 - 2018/12/1
N2 - We performed multi-b and multi-diffusion-time diffusion-weighted magnetic resonance imaging on aquaporin-4-expressing (AQ) and -non-expressing (noAQ) cells, and demonstrated a clear difference between the signals from the two cell types. The data were interpreted using a two-compartment (intra and extracellular spaces) model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange-times obtained with this model are compared to those measured using an independent optical imaging technique (coherent anti-Stokes Raman scattering imaging, CARS). For both techniques it was found that the exchange-time estimated for the noAQ cells was significantly longer than that for the AQ cells.
AB - We performed multi-b and multi-diffusion-time diffusion-weighted magnetic resonance imaging on aquaporin-4-expressing (AQ) and -non-expressing (noAQ) cells, and demonstrated a clear difference between the signals from the two cell types. The data were interpreted using a two-compartment (intra and extracellular spaces) model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange-times obtained with this model are compared to those measured using an independent optical imaging technique (coherent anti-Stokes Raman scattering imaging, CARS). For both techniques it was found that the exchange-time estimated for the noAQ cells was significantly longer than that for the AQ cells.
UR - http://www.scopus.com/inward/record.url?scp=85058759783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058759783&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-36264-9
DO - 10.1038/s41598-018-36264-9
M3 - Article
C2 - 30560905
AN - SCOPUS:85058759783
VL - 8
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 17954
ER -