Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells

Takayuki Obata, Jeff Kershaw, Yasuhiko Tachibana, Takayuki Miyauchi, Yoichiro Abe, Sayaka Shibata, Hiroshi Kawaguchi, Yoko Ikoma, Hiroyuki Takuwa, Ichio Aoki, Masato Yasui

Research output: Contribution to journalArticle

Abstract

We performed multi-b and multi-diffusion-time diffusion-weighted magnetic resonance imaging on aquaporin-4-expressing (AQ) and -non-expressing (noAQ) cells, and demonstrated a clear difference between the signals from the two cell types. The data were interpreted using a two-compartment (intra and extracellular spaces) model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange-times obtained with this model are compared to those measured using an independent optical imaging technique (coherent anti-Stokes Raman scattering imaging, CARS). For both techniques it was found that the exchange-time estimated for the noAQ cells was significantly longer than that for the AQ cells.

Original languageEnglish
Article number17954
JournalScientific reports
Volume8
Issue number1
DOIs
Publication statusPublished - 2018 Dec 1

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Aquaporin 4
Diffusion Magnetic Resonance Imaging
Raman Spectrum Analysis
Water
Space Simulation
Optical Imaging
Extracellular Space

ASJC Scopus subject areas

  • General

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Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells. / Obata, Takayuki; Kershaw, Jeff; Tachibana, Yasuhiko; Miyauchi, Takayuki; Abe, Yoichiro; Shibata, Sayaka; Kawaguchi, Hiroshi; Ikoma, Yoko; Takuwa, Hiroyuki; Aoki, Ichio; Yasui, Masato.

In: Scientific reports, Vol. 8, No. 1, 17954, 01.12.2018.

Research output: Contribution to journalArticle

Obata, Takayuki ; Kershaw, Jeff ; Tachibana, Yasuhiko ; Miyauchi, Takayuki ; Abe, Yoichiro ; Shibata, Sayaka ; Kawaguchi, Hiroshi ; Ikoma, Yoko ; Takuwa, Hiroyuki ; Aoki, Ichio ; Yasui, Masato. / Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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AU - Shibata, Sayaka

AU - Kawaguchi, Hiroshi

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