Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay and by the Friedewald formula in a large community population

Kozo Tanno, Tomonori Okamura, Masaki Ohsawa, Toshiyuki Onoda, Kazuyoshi Itai, Kiyomi Sakata, Motoyuki Nakamura, Akira Ogawa, Kazuko Kawamura, Akira Okayama

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: We compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population. Methods: A total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52 mmol/l were enrolled. LDL-C were directly measured by the enzymatic homogeneous assay (LDL-C (D)) and also estimated by the FF (LDL-C (F)). Paired t-test, Pearson's correlation coefficient and linear regression analysis were performed and the concordances of the National Cholesterol Education Program (NCEP) risk category were estimated. Results: Both in fasting (n. = 3270) and nonfasting samples (n. = 17,924), LDL-C (D) highly correlated with LDL-C (F): r= 0.971 and 0.955, respectively. Concordant results for NCEP categories were 84.8% for fasting samples and 80.1% for nonfasting samples. However, the bias between the 2 measurements increased in samples with TG concentrations > 1.69 mmol/l, especially in nonfasting samples. Conclusions: The results showing less variability of the direct LDL-C assay than that of the FF in nonfasting samples suggest that epidemiological studies can use LDL-C measured by the direct assay both in fasting and nonfasting samples.

Original languageEnglish
Pages (from-to)1774-1780
Number of pages7
JournalClinica Chimica Acta
Volume411
Issue number21-22
DOIs
Publication statusPublished - 2010 Nov
Externally publishedYes

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LDL Cholesterol
Assays
Population
Independent Living
Epidemiologic Studies
Fasting
Healthy Volunteers
Triglycerides

Keywords

  • Community-based study
  • Enzymatic homogeneous LDL-C assay
  • Friedewald formula
  • Japanese
  • Low-density lipoprotein cholesterol
  • Triglyceride

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay and by the Friedewald formula in a large community population. / Tanno, Kozo; Okamura, Tomonori; Ohsawa, Masaki; Onoda, Toshiyuki; Itai, Kazuyoshi; Sakata, Kiyomi; Nakamura, Motoyuki; Ogawa, Akira; Kawamura, Kazuko; Okayama, Akira.

In: Clinica Chimica Acta, Vol. 411, No. 21-22, 11.2010, p. 1774-1780.

Research output: Contribution to journalArticle

Tanno, Kozo ; Okamura, Tomonori ; Ohsawa, Masaki ; Onoda, Toshiyuki ; Itai, Kazuyoshi ; Sakata, Kiyomi ; Nakamura, Motoyuki ; Ogawa, Akira ; Kawamura, Kazuko ; Okayama, Akira. / Comparison of low-density lipoprotein cholesterol concentrations measured by a direct homogeneous assay and by the Friedewald formula in a large community population. In: Clinica Chimica Acta. 2010 ; Vol. 411, No. 21-22. pp. 1774-1780.
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AU - Onoda, Toshiyuki

AU - Itai, Kazuyoshi

AU - Sakata, Kiyomi

AU - Nakamura, Motoyuki

AU - Ogawa, Akira

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AU - Okayama, Akira

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N2 - Background: We compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population. Methods: A total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52 mmol/l were enrolled. LDL-C were directly measured by the enzymatic homogeneous assay (LDL-C (D)) and also estimated by the FF (LDL-C (F)). Paired t-test, Pearson's correlation coefficient and linear regression analysis were performed and the concordances of the National Cholesterol Education Program (NCEP) risk category were estimated. Results: Both in fasting (n. = 3270) and nonfasting samples (n. = 17,924), LDL-C (D) highly correlated with LDL-C (F): r= 0.971 and 0.955, respectively. Concordant results for NCEP categories were 84.8% for fasting samples and 80.1% for nonfasting samples. However, the bias between the 2 measurements increased in samples with TG concentrations > 1.69 mmol/l, especially in nonfasting samples. Conclusions: The results showing less variability of the direct LDL-C assay than that of the FF in nonfasting samples suggest that epidemiological studies can use LDL-C measured by the direct assay both in fasting and nonfasting samples.

AB - Background: We compare the direct homogeneous low-density lipoprotein cholesterol (LDL-C) assay with the Friedewald formula (FF) for determination of LDL-C in a large community-dwelling population. Methods: A total of 21,194 apparently healthy subjects aged 40 to 79 years with triglyceride (TG) concentrations < 4.52 mmol/l were enrolled. LDL-C were directly measured by the enzymatic homogeneous assay (LDL-C (D)) and also estimated by the FF (LDL-C (F)). Paired t-test, Pearson's correlation coefficient and linear regression analysis were performed and the concordances of the National Cholesterol Education Program (NCEP) risk category were estimated. Results: Both in fasting (n. = 3270) and nonfasting samples (n. = 17,924), LDL-C (D) highly correlated with LDL-C (F): r= 0.971 and 0.955, respectively. Concordant results for NCEP categories were 84.8% for fasting samples and 80.1% for nonfasting samples. However, the bias between the 2 measurements increased in samples with TG concentrations > 1.69 mmol/l, especially in nonfasting samples. Conclusions: The results showing less variability of the direct LDL-C assay than that of the FF in nonfasting samples suggest that epidemiological studies can use LDL-C measured by the direct assay both in fasting and nonfasting samples.

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