Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas

Johannes Riegler, Antje Ebert, Xulei Qin, Qi Shen, Mouer Wang, Mohamed Ameen, Kazuki Kodo, Sang Ging Ong, Won Hee Lee, Grace Lee, Evgenios Neofytou, Joseph D. Gold, Andrew J. Connolly, Joseph C. Wu

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14 Citations (Scopus)

Abstract

The use of cells derived from pluripotent stem cells (PSCs) for regenerative therapies confers a considerable risk for neoplastic growth and teratoma formation. Preclinical and clinical assessment of such therapies will require suitable monitoring strategies to understand and mitigate these risks. Here we generated human-induced pluripotent stem cells (iPSCs), selected clones that continued to express reprogramming factors after differentiation into cardiomyocytes, and transplanted these cardiomyocytes into immunocompromised rat hearts post-myocardial infarction. We compared magnetic resonance imaging (MRI), cardiac ultrasound, and serum biomarkers for their ability to delineate teratoma formation and growth. MRI enabled the detection of teratomas with a volume >8 mm3. A combination of three plasma biomarkers (CEA, AFP, and HCG) was able to detect teratomas with a volume >17 mm3 and with a sensitivity of more than 87%. Based on our findings, a combination of serum biomarkers with MRI screening may offer the highest sensitivity for teratoma detection and tracking.

Original languageEnglish
Pages (from-to)176-187
Number of pages12
JournalStem Cell Reports
Volume6
Issue number2
DOIs
Publication statusPublished - 2016 Feb 9
Externally publishedYes

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Keywords

  • magnetic resonance imaging
  • microRNA biomarker
  • pluripotent stem cells
  • serum biomarker
  • tumorigenicity
  • ultrasound imaging

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Riegler, J., Ebert, A., Qin, X., Shen, Q., Wang, M., Ameen, M., Kodo, K., Ong, S. G., Lee, W. H., Lee, G., Neofytou, E., Gold, J. D., Connolly, A. J., & Wu, J. C. (2016). Comparison of Magnetic Resonance Imaging and Serum Biomarkers for Detection of Human Pluripotent Stem Cell-Derived Teratomas. Stem Cell Reports, 6(2), 176-187. https://doi.org/10.1016/j.stemcr.2015.12.008