Comparison of Sexual Reproductive Behaviors in Two Species of Macrobiotidae (Tardigrada: Eutardigrada)

Kenta Sugiura, Hiroki Minato, Atsushi C Suzuki, Kazuharu Arakawa, Takekazu Kunieda, Midori Matsumoto

Research output: Contribution to journalArticle

Abstract

Reproductive strategy is an important aspect of biological diversity. In tardigrades, several reproductive modes, including sexual reproduction, are known. However, tardigrade mating behavior has been observed only rarely in most species, and in some cases, especially in the freely ovipositing eutardigrades, remains entirely unknown. In the present study, we cultured two sexually reproducing tardigrade species that lay eggs freely, Paramacrobiotus sp. TYO strain and Macrobiotus shonaicus, to investigate and compare their courtship, mating, and chromosome morphology. Mating behavior was observed and recorded in both species. The entire mating sequence, including courtship, was categorized into five discrete steps common to two species, as follows: [1] Tracking: the male tracks and orientates toward the female; [2] Touching: the male makes contact with the cloaca of the female; [3] Standstill: the female ceases movement until male ejaculation is complete; [4] Ejaculation: the male curls its caudal end and ejaculates into the cloaca from close range; [5] Contraction: the female contracts its ventral side after ejaculation to capture spermatozoa deposited in the external environment in close proximity to the cloaca. Some notable differences between the two species were observed in the steps 3-4. First, oviposition was observed at 40 min in Paramacrobiotus sp. TYO strain, and a few days after mating in M. shonaicus, respectively. Comparisons of chromosome morphology before and after mating indicated that oocytes are arrested at metaphase I in both species. Spermatozoa attach to the interior of the chorion of laid eggs.

Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalZoological Science
Volume36
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

Tardigrada
sexual behavior
reproductive behavior
cloaca
ejaculation
chromosome morphology
mating behavior
courtship
spermatozoa
chorion
sexual reproduction
metaphase
oocytes
oviposition
biodiversity

Keywords

  • chromosomal analyses
  • Macrobiotus shonaicus
  • mating behavior
  • Paramacrobiotus sp.
  • sexual reproduction

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Comparison of Sexual Reproductive Behaviors in Two Species of Macrobiotidae (Tardigrada : Eutardigrada). / Sugiura, Kenta; Minato, Hiroki; Suzuki, Atsushi C; Arakawa, Kazuharu; Kunieda, Takekazu; Matsumoto, Midori.

In: Zoological Science, Vol. 36, No. 2, 01.01.2019, p. 120-127.

Research output: Contribution to journalArticle

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