Comparison of telomere length and association with progenitor cell markers in lacrimal gland between sjögren syndrome and non-sjögren syndrome dry eye patients

Motoko Kawashima, Tetsuya Kawakita, Yoshiko Maida, Mizuka Kamoi, Yoko Ogawa, Shigeto Shimmura, Kenkichi Masutomi, Kazuo Tsubota

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridization of telomeres (telo-FISH) for the assessment of telomere length in lacrimal gland in Sjögren and non- Sjögren syndrome patients; and 2) investigate the relationship between progenitor cell markers and telomere length in both groups. Methods: Quantitative fluorescence in situ hybridization with a peptide nucleic acid probe complementary to the telomere repeat sequence was performed on frozen sections from human lacrimal gland tissues. The mean fluorescence intensity of telomere spots was automatically quantified by image analysis as relative telomere length in lacrimal gland epithelial cells. Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed. Results: Telomere intensity in the Sjögren syndrome group (6,785.0±455) was significantly lower than that in the non- Sjögren syndrome group (7,494.7±477; p=0.02). Among the samples from the non-Sjögren syndrome group, immunostaining revealed that p63 was expressed in 1-3 acinar cells in each acinar unit and continuously in the basal layer of duct cells. In contrast, in the Sjögren syndrome group, p63 and nucleostemin showed a lower level of expression. ABCG2 was expressed in acinar cells in both sjogren and non-Sjogren syndrome. Conclusions: The results of this study indicate that 1) telo-FISH is a viable method of assessing telomere length in lacrimal gland, and 2) telomere length in Sjögren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjögren syndrome.

Original languageEnglish
Pages (from-to)1397-1404
Number of pages8
JournalMolecular Vision
Volume17
Publication statusPublished - 2011

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Dry Eye Syndromes
Lacrimal Apparatus
Telomere
Stem Cells
Acinar Cells
Fluorescence In Situ Hybridization
Adenosine Triphosphate
Nucleic Acid Probes
Peptide Nucleic Acids
Nestin
Frozen Sections
Fluorescence
Epithelial Cells

ASJC Scopus subject areas

  • Ophthalmology

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Comparison of telomere length and association with progenitor cell markers in lacrimal gland between sjögren syndrome and non-sjögren syndrome dry eye patients. / Kawashima, Motoko; Kawakita, Tetsuya; Maida, Yoshiko; Kamoi, Mizuka; Ogawa, Yoko; Shimmura, Shigeto; Masutomi, Kenkichi; Tsubota, Kazuo.

In: Molecular Vision, Vol. 17, 2011, p. 1397-1404.

Research output: Contribution to journalArticle

Kawashima, Motoko ; Kawakita, Tetsuya ; Maida, Yoshiko ; Kamoi, Mizuka ; Ogawa, Yoko ; Shimmura, Shigeto ; Masutomi, Kenkichi ; Tsubota, Kazuo. / Comparison of telomere length and association with progenitor cell markers in lacrimal gland between sjögren syndrome and non-sjögren syndrome dry eye patients. In: Molecular Vision. 2011 ; Vol. 17. pp. 1397-1404.
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abstract = "Purpose: Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridization of telomeres (telo-FISH) for the assessment of telomere length in lacrimal gland in Sj{\"o}gren and non- Sj{\"o}gren syndrome patients; and 2) investigate the relationship between progenitor cell markers and telomere length in both groups. Methods: Quantitative fluorescence in situ hybridization with a peptide nucleic acid probe complementary to the telomere repeat sequence was performed on frozen sections from human lacrimal gland tissues. The mean fluorescence intensity of telomere spots was automatically quantified by image analysis as relative telomere length in lacrimal gland epithelial cells. Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed. Results: Telomere intensity in the Sj{\"o}gren syndrome group (6,785.0±455) was significantly lower than that in the non- Sj{\"o}gren syndrome group (7,494.7±477; p=0.02). Among the samples from the non-Sj{\"o}gren syndrome group, immunostaining revealed that p63 was expressed in 1-3 acinar cells in each acinar unit and continuously in the basal layer of duct cells. In contrast, in the Sj{\"o}gren syndrome group, p63 and nucleostemin showed a lower level of expression. ABCG2 was expressed in acinar cells in both sjogren and non-Sjogren syndrome. Conclusions: The results of this study indicate that 1) telo-FISH is a viable method of assessing telomere length in lacrimal gland, and 2) telomere length in Sj{\"o}gren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sj{\"o}gren syndrome.",
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T1 - Comparison of telomere length and association with progenitor cell markers in lacrimal gland between sjögren syndrome and non-sjögren syndrome dry eye patients

AU - Kawashima, Motoko

AU - Kawakita, Tetsuya

AU - Maida, Yoshiko

AU - Kamoi, Mizuka

AU - Ogawa, Yoko

AU - Shimmura, Shigeto

AU - Masutomi, Kenkichi

AU - Tsubota, Kazuo

PY - 2011

Y1 - 2011

N2 - Purpose: Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridization of telomeres (telo-FISH) for the assessment of telomere length in lacrimal gland in Sjögren and non- Sjögren syndrome patients; and 2) investigate the relationship between progenitor cell markers and telomere length in both groups. Methods: Quantitative fluorescence in situ hybridization with a peptide nucleic acid probe complementary to the telomere repeat sequence was performed on frozen sections from human lacrimal gland tissues. The mean fluorescence intensity of telomere spots was automatically quantified by image analysis as relative telomere length in lacrimal gland epithelial cells. Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed. Results: Telomere intensity in the Sjögren syndrome group (6,785.0±455) was significantly lower than that in the non- Sjögren syndrome group (7,494.7±477; p=0.02). Among the samples from the non-Sjögren syndrome group, immunostaining revealed that p63 was expressed in 1-3 acinar cells in each acinar unit and continuously in the basal layer of duct cells. In contrast, in the Sjögren syndrome group, p63 and nucleostemin showed a lower level of expression. ABCG2 was expressed in acinar cells in both sjogren and non-Sjogren syndrome. Conclusions: The results of this study indicate that 1) telo-FISH is a viable method of assessing telomere length in lacrimal gland, and 2) telomere length in Sjögren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjögren syndrome.

AB - Purpose: Indicators of aging such as disruption of telomeric function due to shortening may be more frequent in dysfunctional lacrimal gland. The aims of this study were to 1) determine the viability of quantitative fluorescence in situ hybridization of telomeres (telo-FISH) for the assessment of telomere length in lacrimal gland in Sjögren and non- Sjögren syndrome patients; and 2) investigate the relationship between progenitor cell markers and telomere length in both groups. Methods: Quantitative fluorescence in situ hybridization with a peptide nucleic acid probe complementary to the telomere repeat sequence was performed on frozen sections from human lacrimal gland tissues. The mean fluorescence intensity of telomere spots was automatically quantified by image analysis as relative telomere length in lacrimal gland epithelial cells. Immunostaining for p63, nucleostemin, ATP-binding cassette, sub-family G, member 2 (ABCG2), and nestin was also performed. Results: Telomere intensity in the Sjögren syndrome group (6,785.0±455) was significantly lower than that in the non- Sjögren syndrome group (7,494.7±477; p=0.02). Among the samples from the non-Sjögren syndrome group, immunostaining revealed that p63 was expressed in 1-3 acinar cells in each acinar unit and continuously in the basal layer of duct cells. In contrast, in the Sjögren syndrome group, p63 and nucleostemin showed a lower level of expression. ABCG2 was expressed in acinar cells in both sjogren and non-Sjogren syndrome. Conclusions: The results of this study indicate that 1) telo-FISH is a viable method of assessing telomere length in lacrimal gland, and 2) telomere length in Sjögren syndrome is shorter and associated with lower levels of expression of p63 and nucleostemin than in non-Sjögren syndrome.

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