Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser doppler flowmetry

Masashi Kusano, Haruka Nakayama, Hiroyuki Takuwa, Kazuto Masamoto, Iwao Kanno, Eiji Okada

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Cerebral blood flow (CBF) change of mice during whisker stimulation is measured by laser speckle flowgraphy (LSFG) and laser Doppler flowmetry (LDF). Laser speckle flowgraphy (LSFG) has been used to obtain the two-dimensional distribution of the blood flow in tissue as well as scanning laser Doppler flowmetry (LDF). There are several parameters of LSFG to obtain the blood flow maps and the distribution change of blood flow. In this study, we calculate four parameters from the speckle pattern to measure CBF in awake mice. The temporal resolution of LSFG is likely to be less than that of LDF. The temporal changes in CBF obtained from the four parameters calculated from the speckle pattern detected by a common CCD camera are compared with those measured by LDF. The time courses of CBF change measured by LSFG highly correlate with those by LDF. The results indicate that the temporal response of LSFG is sufficient to measure CBF change evoked by brain activations.

    Original languageEnglish
    Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2011
    PublisherOptical Society of America (OSA)
    ISBN (Print)9780819486837
    DOIs
    Publication statusPublished - 2011 Jan 1
    EventEuropean Conference on Biomedical Optics, ECBO 2011 - Munich, Germany
    Duration: 2011 May 222011 May 26

    Publication series

    NameOptics InfoBase Conference Papers
    ISSN (Electronic)2162-2701

    Other

    OtherEuropean Conference on Biomedical Optics, ECBO 2011
    CountryGermany
    CityMunich
    Period11/5/2211/5/26

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    Keywords

    • Cerebral blood flow
    • Laser doppler flowmetry
    • Laser speckle flowgraphy
    • Optical imaging

    ASJC Scopus subject areas

    • Instrumentation
    • Atomic and Molecular Physics, and Optics

    Cite this

    Kusano, M., Nakayama, H., Takuwa, H., Masamoto, K., Kanno, I., & Okada, E. (2011). Comparison of temporal response to cerebral blood flow measured by laser speckle flowgraphy and laser doppler flowmetry. In European Conference on Biomedical Optics, ECBO 2011 [80880E] (Optics InfoBase Conference Papers). Optical Society of America (OSA). https://doi.org/10.1364/ecbo.2011.80880e