Comparison of the Japan Society of Clinical Chemistry reference method and CDC method for HDL and LDL cholesterol measurements using fresh sera

Yuzo Kayamori, Masakazu Nakamura, Koji Kishi, Takashi Miida, Kunihiro Nishimura, Tomonori Okamura, Satoshi Hirayama, Hirotoshi Ohmura, Hiroshi Yoshida, Masumi Ai, Akira Tanaka, Hiroyuki Sumino, Masami Murakami, Ikuo Inoue, Tamio Teramoto, Shinji Yokoyama

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: In 2009, the Japan Society of Clinical Chemistry (JSCC) recommended a reference method for the measurement of serum high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) levels. This automated method uses cholesterol esterase-cholesterol dehydrogenase to measure cholesterol levels in fractions obtained after ultracentrifugation and dextran sulfate/magnesium chloride precipitation. In the present study, using fresh samples, we compared the LDL-C and HDL-C levels measured using this method with those measured using the traditional Centers for Disease Control and Prevention (CDC)-beta-quantification (BQ) method. Design: and methods: Using both the JSCC and CDC-BQ methods, LDL-C/HDL-C levels were measured in 47 non-diseased and 126 diseased subjects, whose triglyceride levels were lower than 11.29 ​mmol/L (1000 ​mg/dL). Results: For LDL-C, the equation of the line representing the correlation between the two methods was y ​= ​0.991x + 0.009 ​mmol/L; r ​= ​0.999; and Sy/x ​= ​0.025 ​mmol/L, where x is the mean LDL-C level measured using the CDC-BQ method. Similarly, for HDL-C, the equation of the line representing the correlation between the two methods was y ​= ​0.988x + 0.041 ​mmol/L, r ​= ​0.999, and Sy/x ​= ​0.019 ​mmol/L, where x is the mean HDL-C level measured using the CDC-BQ method. Conclusions: The JSCC method agreed with the CDC-BQ method in cases of both non-diseased and diseased subjects, including those with dyslipidemia.

Original languageEnglish
Article numbere00228
JournalPractical Laboratory Medicine
Volume25
DOIs
Publication statusPublished - 2021 May
Externally publishedYes

Keywords

  • Beta-quantification
  • Cholesterol dehydrogenase
  • HDL-cholesterol
  • Homogeneous assay
  • LDL-cholesterol
  • Reference method

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

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