Comparison of various bone marrow fractions in the ability to participate in vascular remodeling after mechanical injury

Makoto Sahara, Masataka Sata, Yumi Matsuzaki, Kimie Tanaka, Toshihiro Morita, Yasunobu Hirata, Hideyuki Okano, Ryozo Nagai

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In contrast to conventional assumption, recent reports propose the possibility that hematopoietic stem cells (HSCs) may have broader potential to differentiate into various cell types. Here, we tested the pluripotency of HSCs by comparing vascular lesions induced by mechanical injury after bone marrow reconstitution with total bone marrow (TBM) cells, C-KH+ Sca-1 + Lin- (KSL) cells, or a single HSC cell (Tip-SP CD34-KSL cell, CD34- c-Kit+ Sca-1+ Lin- cell with the strongest dyeefflux activity) harboring green fluorescent protein (GFP). The lesions contained a significant number of GFP-positive cells in the TBM and KSL groups, whereas GFP-positive cells were rarely detected in the HSC group. These results suggest that transdifferentiation of a highly purified HSC seems to be a rare event, if it occurs at all, whereas bone marrow cells including the KSL fraction can give rise to vascular cells that substantially contribute to repair or lesion formation after mechanical injury.

Original languageEnglish
Pages (from-to)874-878
Number of pages5
JournalStem Cells
Volume23
Issue number7
DOIs
Publication statusPublished - 2005 Aug 1

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Keywords

  • Endothelial cells
  • Hematopoietic stem cells
  • Progenitor
  • Smooth muscle cells
  • Transdifferentiation

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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