Compartmentalization of Peyer's patch anlagen before lymphocyte entry

H. Hashi, H. Yoshida, Kenya Honda, S. Fraser, H. Kubo, M. Awane, A. Takabayashi, H. Nakano, Y. Yamaoka, S. I. Nishikawa

Research output: Contribution to journalArticle

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Abstract

We have shown that Peyer's patch (PP) first develops as a simple and even cell aggregation during embryogenesis. To investigate when and how such a simple cell aggregation forms the complex PP architecture, we analyzed the distribution of cells expressing IL-7Rα (PP inducer cells), VCAM-1 (mesenchymal cells), CD11c (dendritic cells), and mature lymphocytes by whole-mount immunostaining of 17.5 days postcoitus to 2 days postpartum mouse gut. Our results show that compartmentalization of PP anlagen commences at day 18.5 of gestation by clustering and subsequent follicle formation of IL-7Rα+, VCAM-1+, and CD11c+ cells. This process adds the primitive architecture of PP anlage with several follicles in which IL-7Rα+ cells localize in the center, while VCAM-1+ and CD11c+ cells localize at the fringe. This follicle formation is accompanied by the establishment of PP-specific vascular network expressing mucosal addressin cellular adhesion molecule-1. Mature B and T lymphocytes entering in the PP anlage are distributed promptly to their own target zones; B cells to the follicle and T cells to nonfollicular zones. Our analysis of scid/scid mouse indicate that the initial processes including formation of PP-specific vascular network occur in the absence of lymphocytes. These observations indicate that the basic architecture of PP is formed by a set of cell lineages assembled during the initial phase of induction of PP anlagen before entry of mature lymphocytes.

Original languageEnglish
Pages (from-to)3702-3709
Number of pages8
JournalJournal of Immunology
Volume166
Issue number6
Publication statusPublished - 2001 Mar 15
Externally publishedYes

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Peyer's Patches
Lymphocytes
Vascular Cell Adhesion Molecule-1
Cell Aggregation
Blood Vessels
B-Lymphocytes
T-Lymphocytes
Cell Lineage
Helper-Inducer T-Lymphocytes
Dendritic Cells
Postpartum Period
Embryonic Development
Cluster Analysis

ASJC Scopus subject areas

  • Immunology

Cite this

Hashi, H., Yoshida, H., Honda, K., Fraser, S., Kubo, H., Awane, M., ... Nishikawa, S. I. (2001). Compartmentalization of Peyer's patch anlagen before lymphocyte entry. Journal of Immunology, 166(6), 3702-3709.

Compartmentalization of Peyer's patch anlagen before lymphocyte entry. / Hashi, H.; Yoshida, H.; Honda, Kenya; Fraser, S.; Kubo, H.; Awane, M.; Takabayashi, A.; Nakano, H.; Yamaoka, Y.; Nishikawa, S. I.

In: Journal of Immunology, Vol. 166, No. 6, 15.03.2001, p. 3702-3709.

Research output: Contribution to journalArticle

Hashi, H, Yoshida, H, Honda, K, Fraser, S, Kubo, H, Awane, M, Takabayashi, A, Nakano, H, Yamaoka, Y & Nishikawa, SI 2001, 'Compartmentalization of Peyer's patch anlagen before lymphocyte entry', Journal of Immunology, vol. 166, no. 6, pp. 3702-3709.
Hashi H, Yoshida H, Honda K, Fraser S, Kubo H, Awane M et al. Compartmentalization of Peyer's patch anlagen before lymphocyte entry. Journal of Immunology. 2001 Mar 15;166(6):3702-3709.
Hashi, H. ; Yoshida, H. ; Honda, Kenya ; Fraser, S. ; Kubo, H. ; Awane, M. ; Takabayashi, A. ; Nakano, H. ; Yamaoka, Y. ; Nishikawa, S. I. / Compartmentalization of Peyer's patch anlagen before lymphocyte entry. In: Journal of Immunology. 2001 ; Vol. 166, No. 6. pp. 3702-3709.
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