Compartmentalized Spherical Collagen Microparticles for Anisotropic Cell Culture Microenvironments

Satoru Yoshida, Masahiro Takinoue, Hiroaki Onoe

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This paper describes a new fabrication method for obtaining anisotropic spherical hydrogel microparticles with different types of extracellular matrix (ECM) hemispheres for use in 3D cell culture. To fabricate the microparticles, a mixture of an ECM precursor solution and sodium alginate is ejected into a calcium chloride solution under large centrifugal acceleration by a centrifuge-based microfluidic device; the calcium alginate hydrogel plays a significant role as a "sacrificial gelation template" to maintain the ECM molecules in each hemisphere. This fabrication method enables gaining control of the hemispherical volume, density, and type of ECM. Using the microparticles, cells could be successfully encapsulated in each hemisphere selectively with high viability, which are then suitable for culture in the microparticles to form microtissues. It is believed that the proposed anisotropic ECM microparticles will facilitate the coculture of multiple cell types in different ECMs, which is similar to in vivo microenvironments, facilitating control of cell behavior in an anisotropic microenvironment for the benefit of large-scale and quantitative analyses in vitro.

Original languageEnglish
JournalAdvanced healthcare materials
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Cellular Microenvironment
Hydrogel
Cell culture
Collagen
Hydrogels
Extracellular Matrix
Cell Culture Techniques
Military electronic countermeasures
Fabrication
Sodium alginate
Calcium Chloride
Calcium chloride
Alginate
Centrifuges
Gelation
Microfluidics
Calcium
Lab-On-A-Chip Devices
Molecules
Behavior Control

Keywords

  • Anisotropic microenvironments
  • Calcium alginate hydrogel
  • Centrifuge-based microfluidic device
  • Collagen
  • Microparticles

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Compartmentalized Spherical Collagen Microparticles for Anisotropic Cell Culture Microenvironments. / Yoshida, Satoru; Takinoue, Masahiro; Onoe, Hiroaki.

In: Advanced healthcare materials, 2017.

Research output: Contribution to journalArticle

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