Abstract
The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequence around the GC boxes with bisulphite sequencing. In agreement with restriction site analysis by other labs, we found preferential demethylation at GC box DNA versus control DNA. However, the bisulphite sequencing which permits the analysis of every CpG site on a given DNA molecule, revealed a complex pattern of methylated and unmethylated sites. Upon prolonged culture the pattern became simpler, with most sites demethylated but certain sites being consistently methylated.
Original language | English |
---|---|
Pages (from-to) | 170-174 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 370 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1995 Aug 21 |
Externally published | Yes |
Keywords
- Bisulphite PCR
- DNA methylation
- Transcription factor
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology