TY - JOUR
T1 - Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia
AU - Hamano-Hasegawa, Keiko
AU - Morozumi, Miyuki
AU - Nakayama, Eiichi
AU - Chiba, Naoko
AU - Murayama, Somay Y.
AU - Takayanagi, Reiko
AU - Iwata, Satoshi
AU - Sunakawa, Keisuke
AU - Ubukata, Kimiko
AU - Ishizawa, Shinobu
AU - Numata, Mika
AU - Kojima, Michiyo
AU - Nonoyama, Masato
AU - Kuroki, Haruo
AU - Shimizu, Koichi
AU - Kobayashi, Masaaki
AU - Saito, Kota
AU - Nitta, Masahiko
AU - Kawamura, Naohisa
AU - Sakai, Ritsuko
AU - Ohnari, Shigeru
AU - Morinobu, Takao
AU - Tajima, Takeshi
AU - Oishi, Tomohiro
PY - 2008/12
Y1 - 2008/12
N2 - We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13 respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and B; parainfluenza virus (PIV) 1, 2, and 3; adenovirus; rhinovirus (RV); enterovirus; coronavirus (OC43); human metapneumovirus (hMPV); and human bocavirus (HBoV). The new method for detection of these viruses was applied simultaneously with real-time PCR for the detection of six bacterial pathogens in clinical samples from 1700 pediatric patients with community-acquired pneumonia (CAP). Of all the patients, 32.5% were suspected to have single bacterial infections; 1.9%, multiple bacterial infections; 15.2%, coinfections of bacteria and viruses; 25.8%, single viral infections; and 2.1%, multiple viral infections. In the remaining 22.6%, the etiology was unknown. The breakdown of suspected causative pathogens was as follows: 24.4% were Streptococcus pneumoniae, 14.8% were Mycoplasma pneumoniae, 11.3% were Haemophilus influenzae, and 1.4% were Chlamydophila pneumoniae. The breakdown of viruses was as follows: 14.5% were RV, 9.4% were RSV, 7.4% were hMPV, 7.2% were PIV, and 2.9% were HBoV. The new method will contribute to advances in the accuracy of diagnosis and should also result in the appropriate use of antimicrobials.
AB - We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13 respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and B; parainfluenza virus (PIV) 1, 2, and 3; adenovirus; rhinovirus (RV); enterovirus; coronavirus (OC43); human metapneumovirus (hMPV); and human bocavirus (HBoV). The new method for detection of these viruses was applied simultaneously with real-time PCR for the detection of six bacterial pathogens in clinical samples from 1700 pediatric patients with community-acquired pneumonia (CAP). Of all the patients, 32.5% were suspected to have single bacterial infections; 1.9%, multiple bacterial infections; 15.2%, coinfections of bacteria and viruses; 25.8%, single viral infections; and 2.1%, multiple viral infections. In the remaining 22.6%, the etiology was unknown. The breakdown of suspected causative pathogens was as follows: 24.4% were Streptococcus pneumoniae, 14.8% were Mycoplasma pneumoniae, 11.3% were Haemophilus influenzae, and 1.4% were Chlamydophila pneumoniae. The breakdown of viruses was as follows: 14.5% were RV, 9.4% were RSV, 7.4% were hMPV, 7.2% were PIV, and 2.9% were HBoV. The new method will contribute to advances in the accuracy of diagnosis and should also result in the appropriate use of antimicrobials.
KW - Child
KW - Community-acquired pneumonia
KW - Real-time RT-PCR
KW - Respiratory virus
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U2 - 10.1007/s10156-008-0648-6
DO - 10.1007/s10156-008-0648-6
M3 - Article
C2 - 19089556
AN - SCOPUS:57749178863
VL - 14
SP - 424
EP - 432
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
SN - 1341-321X
IS - 6
ER -