Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia

Keiko Hamano-Hasegawa, Miyuki Morozumi, Eiichi Nakayama, Naoko Chiba, Somay Y. Murayama, Reiko Takayanagi, Satoshi Iwata, Keisuke Sunakawa, Kimiko Ubukata, Shinobu Ishizawa, Mika Numata, Michiyo Kojima, Masato Nonoyama, Haruo Kuroki, Koichi Shimizu, Masaaki Kobayashi, Kota Saito, Masahiko Nitta, Naohisa Kawamura, Ritsuko SakaiShigeru Ohnari, Takao Morinobu, Takeshi Tajima, Tomohiro Oishi

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We have developed a real-time reverse transcription-PCR (RT-PCR) method to detect 13 respiratory viruses: influenza virus A and B; respiratory syncytial virus (RSV) subgroup A and B; parainfluenza virus (PIV) 1, 2, and 3; adenovirus; rhinovirus (RV); enterovirus; coronavirus (OC43); human metapneumovirus (hMPV); and human bocavirus (HBoV). The new method for detection of these viruses was applied simultaneously with real-time PCR for the detection of six bacterial pathogens in clinical samples from 1700 pediatric patients with community-acquired pneumonia (CAP). Of all the patients, 32.5% were suspected to have single bacterial infections; 1.9%, multiple bacterial infections; 15.2%, coinfections of bacteria and viruses; 25.8%, single viral infections; and 2.1%, multiple viral infections. In the remaining 22.6%, the etiology was unknown. The breakdown of suspected causative pathogens was as follows: 24.4% were Streptococcus pneumoniae, 14.8% were Mycoplasma pneumoniae, 11.3% were Haemophilus influenzae, and 1.4% were Chlamydophila pneumoniae. The breakdown of viruses was as follows: 14.5% were RV, 9.4% were RSV, 7.4% were hMPV, 7.2% were PIV, and 2.9% were HBoV. The new method will contribute to advances in the accuracy of diagnosis and should also result in the appropriate use of antimicrobials.

Original languageEnglish
Pages (from-to)424-432
Number of pages9
JournalJournal of Infection and Chemotherapy
Volume14
Issue number6
DOIs
Publication statusPublished - 2008 Dec

Keywords

  • Child
  • Community-acquired pneumonia
  • Real-time RT-PCR
  • Respiratory virus

ASJC Scopus subject areas

  • Microbiology (medical)
  • Pharmacology (medical)
  • Infectious Diseases

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  • Cite this

    Hamano-Hasegawa, K., Morozumi, M., Nakayama, E., Chiba, N., Murayama, S. Y., Takayanagi, R., Iwata, S., Sunakawa, K., Ubukata, K., Ishizawa, S., Numata, M., Kojima, M., Nonoyama, M., Kuroki, H., Shimizu, K., Kobayashi, M., Saito, K., Nitta, M., Kawamura, N., ... Oishi, T. (2008). Comprehensive detection of causative pathogens using real-time PCR to diagnose pediatric community-acquired pneumonia. Journal of Infection and Chemotherapy, 14(6), 424-432. https://doi.org/10.1007/s10156-008-0648-6