Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells

Kazutaka Terahara, Masato Yoshida, Osamu Igarashi, Tomonori Nochi, Gemilson Soares Pontes, Kouji Hase, Hiroshi Ohno, Shiho Kurokawa, Mio Mejima, Naoko Takayama, Yoshikazu Yuki, Anson W. Lowe, Hiroshi Kiyono

Research output: Contribution to journalArticle

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Abstract

Separate populations of M cells have been detected in the follicle-associated epithelium of. Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.

Original languageEnglish
Pages (from-to)7840-7846
Number of pages7
JournalJournal of Immunology
Volume180
Issue number12
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Peyer's Patches
Gene Expression Profiling
Epithelial Cells
Cholera Toxin
Epithelium
Gene Expression
Chemokines
Lectins
Population
Small Intestine
Glycoproteins
Proteins
Genome

ASJC Scopus subject areas

  • Immunology

Cite this

Terahara, K., Yoshida, M., Igarashi, O., Nochi, T., Pontes, G. S., Hase, K., ... Kiyono, H. (2008). Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells. Journal of Immunology, 180(12), 7840-7846.

Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells. / Terahara, Kazutaka; Yoshida, Masato; Igarashi, Osamu; Nochi, Tomonori; Pontes, Gemilson Soares; Hase, Kouji; Ohno, Hiroshi; Kurokawa, Shiho; Mejima, Mio; Takayama, Naoko; Yuki, Yoshikazu; Lowe, Anson W.; Kiyono, Hiroshi.

In: Journal of Immunology, Vol. 180, No. 12, 2008, p. 7840-7846.

Research output: Contribution to journalArticle

Terahara, K, Yoshida, M, Igarashi, O, Nochi, T, Pontes, GS, Hase, K, Ohno, H, Kurokawa, S, Mejima, M, Takayama, N, Yuki, Y, Lowe, AW & Kiyono, H 2008, 'Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells', Journal of Immunology, vol. 180, no. 12, pp. 7840-7846.
Terahara, Kazutaka ; Yoshida, Masato ; Igarashi, Osamu ; Nochi, Tomonori ; Pontes, Gemilson Soares ; Hase, Kouji ; Ohno, Hiroshi ; Kurokawa, Shiho ; Mejima, Mio ; Takayama, Naoko ; Yuki, Yoshikazu ; Lowe, Anson W. ; Kiyono, Hiroshi. / Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells. In: Journal of Immunology. 2008 ; Vol. 180, No. 12. pp. 7840-7846.
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