Abstract
Separate populations of M cells have been detected in the follicle-associated epithelium of. Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.
| Original language | English |
|---|---|
| Pages (from-to) | 7840-7846 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 180 |
| Issue number | 12 |
| Publication status | Published - 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells. / Terahara, Kazutaka; Yoshida, Masato; Igarashi, Osamu; Nochi, Tomonori; Pontes, Gemilson Soares; Hase, Kouji; Ohno, Hiroshi; Kurokawa, Shiho; Mejima, Mio; Takayama, Naoko; Yuki, Yoshikazu; Lowe, Anson W.; Kiyono, Hiroshi.
In: Journal of Immunology, Vol. 180, No. 12, 2008, p. 7840-7846.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comprehensive gene expression profiling of peyer's patch m cells, villous m-like cells, and intestinal epithelial cells
AU - Terahara, Kazutaka
AU - Yoshida, Masato
AU - Igarashi, Osamu
AU - Nochi, Tomonori
AU - Pontes, Gemilson Soares
AU - Hase, Kouji
AU - Ohno, Hiroshi
AU - Kurokawa, Shiho
AU - Mejima, Mio
AU - Takayama, Naoko
AU - Yuki, Yoshikazu
AU - Lowe, Anson W.
AU - Kiyono, Hiroshi
PY - 2008
Y1 - 2008
N2 - Separate populations of M cells have been detected in the follicle-associated epithelium of. Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.
AB - Separate populations of M cells have been detected in the follicle-associated epithelium of. Peyer's patches (PPs) and the villous epithelium of the small intestine, but the traits shared by or distinguishing the two populations have not been characterized. Our separate study has demonstrated that a potent mucosal modulator cholera toxin (CT) can induce lectin Ulex europaeus agglutinin-1 and our newly developed M cell-specific mAb NKM 16-2-4-positive M-like cells in the duodenal villous epithelium. In this study, we determined the gene expression of PP M cells, CT-induced villous M-like cells, and intestinal epithelial cells isolated by a novel approach using FACS. Additional mRNA and protein analyses confirmed the specific expression of glycoprotein 2 and myristoylated alanine-rich C kinase substrate (MARCKS)-like protein by PP M cells but not CT-induced villous M-like cells. Comprehensive gene profiling also suggested that CT-induced villous M-like cells share traits of both PP M cells and intestinal epithelial cells, a finding that is supported by their unique expression of specific chemokines. The genome-wide assessment of gene expression facilitates discovery of M cell-specific molecules and enhances the molecular understanding of M cell immunobiology.
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UR - http://www.scopus.com/inward/citedby.url?scp=50949083497&partnerID=8YFLogxK
M3 - Article
C2 - 18523247
AN - SCOPUS:50949083497
VL - 180
SP - 7840
EP - 7846
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 12
ER -