Comprehensive genetic characterization of rosette-forming glioneuronal tumors: Independent component analysis by tissue microdissection

Yohei Kitamura, Takashi Komori, Makoto Shibuya, Kentaro Ohara, Yuko Saito, Saeko Hayashi, Aya Sasaki, Eiji Nakagawa, Ryosuke Tomio, Akiyoshi Kakita, Masashi Nakatsukasa, Kazunari Yoshida, Hikaru Sasaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A rosette-forming glioneuronal tumor (RGNT) is a rare mixed neuronal-glial tumor characterized by biphasic architecture of glial and neurocytic components. The number of reports of genetic analyses of RGNTs is few. Additionally, the genetic background of the unique biphasic pathological characteristics of such mixed neuronal-glial tumors remains unclear. To clarify the genetic background of RGNTs, we performed separate comprehensive genetic analyses of glial and neurocytic components of five RGNTs, by tissue microdissection. Two missense mutations in FGFR1 in both components of two cases, and one mutation in PIK3CA in both components of one case, were detected. In the latter case with PIK3CA mutation, the additional FGFR1 mutation was detected only in the glial component. Moreover, the loss of chromosome 13q in only the neurocytic component was observed in one other case. Their results suggested that RGNTs, which are tumors harboring two divergent differentiations that arose from a single clone, have a diverse genetic background. Although previous studies have suggested that RGNTs and pilocytic astrocytomas (PAs) represent the same tumor entity, their results confirm that the genetic background of RGNTs is not identical to that of PA.

Original languageEnglish
JournalBrain Pathology
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Microdissection
Neuroglia
Astrocytoma
Neoplasms
Mutation
Missense Mutation
Clone Cells
Chromosomes
Genetic Background

Keywords

  • CGH
  • FGFR1
  • Genetics
  • Mixed neuronal-glial tumor
  • PIK3CA
  • Rosette-forming glioneuronal tumor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)
  • Clinical Neurology

Cite this

Comprehensive genetic characterization of rosette-forming glioneuronal tumors : Independent component analysis by tissue microdissection. / Kitamura, Yohei; Komori, Takashi; Shibuya, Makoto; Ohara, Kentaro; Saito, Yuko; Hayashi, Saeko; Sasaki, Aya; Nakagawa, Eiji; Tomio, Ryosuke; Kakita, Akiyoshi; Nakatsukasa, Masashi; Yoshida, Kazunari; Sasaki, Hikaru.

In: Brain Pathology, 2017.

Research output: Contribution to journalArticle

Kitamura, Y, Komori, T, Shibuya, M, Ohara, K, Saito, Y, Hayashi, S, Sasaki, A, Nakagawa, E, Tomio, R, Kakita, A, Nakatsukasa, M, Yoshida, K & Sasaki, H 2017, 'Comprehensive genetic characterization of rosette-forming glioneuronal tumors: Independent component analysis by tissue microdissection', Brain Pathology. https://doi.org/10.1111/bpa.12468
Kitamura, Yohei ; Komori, Takashi ; Shibuya, Makoto ; Ohara, Kentaro ; Saito, Yuko ; Hayashi, Saeko ; Sasaki, Aya ; Nakagawa, Eiji ; Tomio, Ryosuke ; Kakita, Akiyoshi ; Nakatsukasa, Masashi ; Yoshida, Kazunari ; Sasaki, Hikaru. / Comprehensive genetic characterization of rosette-forming glioneuronal tumors : Independent component analysis by tissue microdissection. In: Brain Pathology. 2017.
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