Comprehensive metabolome analysis of intracellular metabolites in cultured cells

Ryuhei Kudo, Kaori Igarashi, Tomoyoshi Soga, Takamasa Ishikawa, Yasuhiro Saito

Research output: Contribution to journalArticlepeer-review

Abstract

Capillary electrophoresis mass spectrometry (CE-MS) can measure the intracellular amount of highly polar and charged metabolites; liquid chromatography mass spectrometry (LC-MS) can quantify hydrophobic metabolites. A comprehensive metabolome analysis requires independent sample preparation for LC-MS and CE-MS. Here, we present a protocol to prepare for sequentially analyzing the metabolites from one sample. Here we describe the steps for breast cancer cell lines, MCF-7 cells, but the protocol can be applied to other cell types.

Original languageEnglish
Article number101531
JournalSTAR Protocols
Volume3
Issue number3
DOIs
Publication statusPublished - 2022 Sep 16

Keywords

  • Cell Biology
  • Cell culture
  • Mass Spectrometry
  • Metabolism
  • Metabolomics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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