Computational analysis suggests a highly bendable, fragile structure for nucleosomal DNA

Tadasu Nozaki, Nozomu Yachie, Ryu Ogawa, Rintaro Saito, Masaru Tomita

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eukaryotic chromosomal DNA coils around histones to form nucleosomes. Although histone affinity for DNA depends on DNA sequence patterns, how nucleosome positioning is determined by them remains unknown. Here, we show relationships between nucleosome positioning and two structural characteristics of DNA conferred by DNA sequence. Analysis of bendability and hydroxyl radical cleavage intensity of nucleosomal DNA sequences indicated that nucleosomal DNA is bendable and fragile and that nucleosome positional stability was correlated with characteristics of DNA. This result explains how histone positioning is partially determined by nucleosomal DNA structure, illuminating the optimization of chromosomal DNA packaging that controls cellular dynamics.

Original languageEnglish
Pages (from-to)10-14
Number of pages5
JournalGene
Volume476
Issue number1-2
DOIs
Publication statusPublished - 2011 May 1

Keywords

  • Bendability
  • Cleavage intensity
  • DNA structure
  • Epigenetics
  • Histone
  • Nucleosome

ASJC Scopus subject areas

  • Genetics

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