Concomitant transcriptional activation of nitric oxide synthase and heme oxygenase genes during nitric oxide-mediated macrophage cytostasis

Shun Ichi Kurata, Midori Matsumoto, Uki Yamashita

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

During in vitro activation of mouse peritoneal macrophages with interferon-γ (IFN-γ) and lipopolysaccharide (LPS), their synthesis of peroxynitrite and their cytostatic activity against mouse lymphocytic leukemia (L1210) cells were examined. The activation of the genes for nitric oxide synthase (iNOS) and heme oxygenase (HO-1) was also determined during the activation of the macrophages. Results showed that activation of peroxynitrite synthesis in macrophages was accompanied by the transcriptional activation of iNOS and HO-1 genes. Both genes seem to be activated simultaneously upon activation of the macrophages. Simultaneous activation of iNOS and HO-1 genes may be important because degradation of heme by HO-1 is one of the most important reaction that produces CO in higher organisms, and nitric oxide (NO) and carbon monoxide (CO) can react with heme-containing guanylate cyclase.

Original languageEnglish
Pages (from-to)49-52
Number of pages4
JournalJournal of Biochemistry
Volume120
Issue number1
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Heme Oxygenase (Decyclizing)
Macrophages
Nitric Oxide Synthase
Transcriptional Activation
Nitric Oxide
Peroxynitrous Acid
Macrophage Activation
Genes
Chemical activation
Carbon Monoxide
Lymphoid Leukemia
Leukemia L1210
Heme Oxygenase-1
Guanylate Cyclase
Peritoneal Macrophages
Cytostatic Agents
Heme
Interferons
Lipopolysaccharides
Degradation

Keywords

  • Carbon monoxide
  • Cytostasis
  • Heme oxygenase
  • Nitric oxide
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Concomitant transcriptional activation of nitric oxide synthase and heme oxygenase genes during nitric oxide-mediated macrophage cytostasis. / Kurata, Shun Ichi; Matsumoto, Midori; Yamashita, Uki.

In: Journal of Biochemistry, Vol. 120, No. 1, 1996, p. 49-52.

Research output: Contribution to journalArticle

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