Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice

T. Araki, M. Shibata, R. Takano, S. Hisahara, S. I. Imamura, Y. Fukuuchi, T. Saruta, H. Okano, M. Miura

Research output: Contribution to journalComment/debatepeer-review

17 Citations (Scopus)

Abstract

p35, a viral inhibitor of caspase, prevents cell death induced by various stimuli. We established an experimental system to study the involvement of caspases in cell death, using primary cultured cells from p35 transgenic mice in which the p35 open reading frame (ORF) had been disrupted by the insertion of a DNA segment flanked by loxP sites, the Cre recognition sites. In this system, p35 expression can be initiated by Cre recombinase. Cardiomyocytes, which are highly sensitive to hypoxic stress, were infected with an adenovirus carrying the cre gene (AxCANCre). Expression of p35 by infection with AxCANCre resulted in inhibition of caspase-3 activation and resistance to hypoxia-induced cell death. Hypoxia-induced cytochrome c release was also attenuated in p35-expressing cardiomyocytes. Our transgenic mice can be used as an experimental model for studying the involvement of caspases in various degenerative diseases as well as programmed cell death both in vitro and in vivo.

Original languageEnglish
Pages (from-to)485-492
Number of pages8
JournalCell Death and Differentiation
Volume7
Issue number5
DOIs
Publication statusPublished - 2000 May

Keywords

  • Cardiomyocytes
  • Caspases
  • Cre/loxP
  • Hypoxia
  • p35

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice'. Together they form a unique fingerprint.

Cite this