Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice

T. Araki, Mamoru Shibata, R. Takano, S. Hisahara, S. I. Imamura, Y. Fukuuchi, T. Saruta, Hideyuki Okano, M. Miura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

p35, a viral inhibitor of caspase, prevents cell death induced by various stimuli. We established an experimental system to study the involvement of caspases in cell death, using primary cultured cells from p35 transgenic mice in which the p35 open reading frame (ORF) had been disrupted by the insertion of a DNA segment flanked by loxP sites, the Cre recognition sites. In this system, p35 expression can be initiated by Cre recombinase. Cardiomyocytes, which are highly sensitive to hypoxic stress, were infected with an adenovirus carrying the cre gene (AxCANCre). Expression of p35 by infection with AxCANCre resulted in inhibition of caspase-3 activation and resistance to hypoxia-induced cell death. Hypoxia-induced cytochrome c release was also attenuated in p35-expressing cardiomyocytes. Our transgenic mice can be used as an experimental model for studying the involvement of caspases in various degenerative diseases as well as programmed cell death both in vitro and in vivo.

Original languageEnglish
Pages (from-to)485-492
Number of pages8
JournalCell Death and Differentiation
Volume7
Issue number5
Publication statusPublished - 2000 May

Fingerprint

Apoptosis Regulatory Proteins
Cardiac Myocytes
Transgenic Mice
Genetic Recombination
Cell Death
DNA
Caspases
Caspase Inhibitors
Cytochromes c
Adenoviridae
Caspase 3
Open Reading Frames
Cultured Cells
Theoretical Models
Infection
Genes
Hypoxia

Keywords

  • Cardiomyocytes
  • Caspases
  • Cre/loxP
  • Hypoxia
  • p35

ASJC Scopus subject areas

  • Cell Biology

Cite this

Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice. / Araki, T.; Shibata, Mamoru; Takano, R.; Hisahara, S.; Imamura, S. I.; Fukuuchi, Y.; Saruta, T.; Okano, Hideyuki; Miura, M.

In: Cell Death and Differentiation, Vol. 7, No. 5, 05.2000, p. 485-492.

Research output: Contribution to journalArticle

Araki, T. ; Shibata, Mamoru ; Takano, R. ; Hisahara, S. ; Imamura, S. I. ; Fukuuchi, Y. ; Saruta, T. ; Okano, Hideyuki ; Miura, M. / Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice. In: Cell Death and Differentiation. 2000 ; Vol. 7, No. 5. pp. 485-492.
@article{536842ea0974407593559d0029b5d724,
title = "Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice",
abstract = "p35, a viral inhibitor of caspase, prevents cell death induced by various stimuli. We established an experimental system to study the involvement of caspases in cell death, using primary cultured cells from p35 transgenic mice in which the p35 open reading frame (ORF) had been disrupted by the insertion of a DNA segment flanked by loxP sites, the Cre recognition sites. In this system, p35 expression can be initiated by Cre recombinase. Cardiomyocytes, which are highly sensitive to hypoxic stress, were infected with an adenovirus carrying the cre gene (AxCANCre). Expression of p35 by infection with AxCANCre resulted in inhibition of caspase-3 activation and resistance to hypoxia-induced cell death. Hypoxia-induced cytochrome c release was also attenuated in p35-expressing cardiomyocytes. Our transgenic mice can be used as an experimental model for studying the involvement of caspases in various degenerative diseases as well as programmed cell death both in vitro and in vivo.",
keywords = "Cardiomyocytes, Caspases, Cre/loxP, Hypoxia, p35",
author = "T. Araki and Mamoru Shibata and R. Takano and S. Hisahara and Imamura, {S. I.} and Y. Fukuuchi and T. Saruta and Hideyuki Okano and M. Miura",
year = "2000",
month = "5",
language = "English",
volume = "7",
pages = "485--492",
journal = "Cell Death and Differentiation",
issn = "1350-9047",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Conditional expression of anti-apoptotic protein p35 by Cre-mediated DNA recombination in cardiomyocytes from loxP-p35-transgenic mice

AU - Araki, T.

AU - Shibata, Mamoru

AU - Takano, R.

AU - Hisahara, S.

AU - Imamura, S. I.

AU - Fukuuchi, Y.

AU - Saruta, T.

AU - Okano, Hideyuki

AU - Miura, M.

PY - 2000/5

Y1 - 2000/5

N2 - p35, a viral inhibitor of caspase, prevents cell death induced by various stimuli. We established an experimental system to study the involvement of caspases in cell death, using primary cultured cells from p35 transgenic mice in which the p35 open reading frame (ORF) had been disrupted by the insertion of a DNA segment flanked by loxP sites, the Cre recognition sites. In this system, p35 expression can be initiated by Cre recombinase. Cardiomyocytes, which are highly sensitive to hypoxic stress, were infected with an adenovirus carrying the cre gene (AxCANCre). Expression of p35 by infection with AxCANCre resulted in inhibition of caspase-3 activation and resistance to hypoxia-induced cell death. Hypoxia-induced cytochrome c release was also attenuated in p35-expressing cardiomyocytes. Our transgenic mice can be used as an experimental model for studying the involvement of caspases in various degenerative diseases as well as programmed cell death both in vitro and in vivo.

AB - p35, a viral inhibitor of caspase, prevents cell death induced by various stimuli. We established an experimental system to study the involvement of caspases in cell death, using primary cultured cells from p35 transgenic mice in which the p35 open reading frame (ORF) had been disrupted by the insertion of a DNA segment flanked by loxP sites, the Cre recognition sites. In this system, p35 expression can be initiated by Cre recombinase. Cardiomyocytes, which are highly sensitive to hypoxic stress, were infected with an adenovirus carrying the cre gene (AxCANCre). Expression of p35 by infection with AxCANCre resulted in inhibition of caspase-3 activation and resistance to hypoxia-induced cell death. Hypoxia-induced cytochrome c release was also attenuated in p35-expressing cardiomyocytes. Our transgenic mice can be used as an experimental model for studying the involvement of caspases in various degenerative diseases as well as programmed cell death both in vitro and in vivo.

KW - Cardiomyocytes

KW - Caspases

KW - Cre/loxP

KW - Hypoxia

KW - p35

UR - http://www.scopus.com/inward/record.url?scp=0034057453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034057453&partnerID=8YFLogxK

M3 - Article

C2 - 10800082

AN - SCOPUS:0034057453

VL - 7

SP - 485

EP - 492

JO - Cell Death and Differentiation

JF - Cell Death and Differentiation

SN - 1350-9047

IS - 5

ER -