Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography: Preclinical longitudinal study in common marmosets

Tsunehiko Konomi, Kanehiro Fujiyoshi, Keigo Hikishima, Yuji Komaki, Osahiko Tsuji, Hirotaka James Okano, Yoshiaki Toyama, Hideyuki Okano, Masaya Nakamura

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Conventional magnetic resonance imaging (MRI) can detect hemorrhage, edema, syrinx, and spinal cord atrophy, but not axonal disruption after spinal cord injury (SCI). We previously demonstrated that diffusion tensor tractography (DTT) could depict axonal disruption after hemisection SCI in common marmosets. In the present study, to determine the relationship between DTT results and functional recovery after contusive SCI, we performed longitudinal DTT, behavioral, and histological analyses before and after contusive SCI in common marmosets. By comparing the tract fiber estimate depicted by DTT with neuronal fibers labeled with RT97 and SMI-31, anti-neurofilament antibodies, we determined the optimal fractional anisotropy (FA) threshold for fiber tracking to be 0.40. The ratio of the number of tract fiber estimates at the lesion site to the number before SCI, determined by DTT, was significantly correlated with the functional recovery after SCI. Moreover, comparison of the longitudinal pre- and post-SCI FA and axial diffusivity (λ||) values revealed that they decreased after injury at the sites caudal to the lesion epicenter in the corticospinal tract and rostral to the lesion epicenter in the dorsal column. The FA values, then, showed partial recovery in the dorsal column. FA-value-oriented color DTT was used to represent axonal sparing or regeneration of the different tracts. These findings indicated that DTT analysis might be a versatile non-invasive tool for evaluating the axonal disruption after SCI.

Original languageEnglish
Pages (from-to)1841-1853
Number of pages13
JournalNeuroImage
Volume63
Issue number4
DOIs
Publication statusPublished - 2012 Dec

Fingerprint

Callithrix
Diffusion Tensor Imaging
Spinal Cord Injuries
Longitudinal Studies
Spinal Cord
Anisotropy
Pyramidal Tracts
Intermediate Filaments
Syringes
Atrophy
Regeneration
Anti-Idiotypic Antibodies
Edema
Color
Magnetic Resonance Imaging
Hemorrhage
Wounds and Injuries

Keywords

  • Common marmoset
  • Diffusion tensor imaging
  • Magnetic resonance imaging
  • Spinal cord injury
  • Tractography

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography : Preclinical longitudinal study in common marmosets. / Konomi, Tsunehiko; Fujiyoshi, Kanehiro; Hikishima, Keigo; Komaki, Yuji; Tsuji, Osahiko; Okano, Hirotaka James; Toyama, Yoshiaki; Okano, Hideyuki; Nakamura, Masaya.

In: NeuroImage, Vol. 63, No. 4, 12.2012, p. 1841-1853.

Research output: Contribution to journalArticle

Konomi, Tsunehiko ; Fujiyoshi, Kanehiro ; Hikishima, Keigo ; Komaki, Yuji ; Tsuji, Osahiko ; Okano, Hirotaka James ; Toyama, Yoshiaki ; Okano, Hideyuki ; Nakamura, Masaya. / Conditions for quantitative evaluation of injured spinal cord by in vivo diffusion tensor imaging and tractography : Preclinical longitudinal study in common marmosets. In: NeuroImage. 2012 ; Vol. 63, No. 4. pp. 1841-1853.
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