Confirmation of bone marrow engraftment by detection of M/N blood group antigens on erythroblasts in erythroid bursts

K. Kitano, E. Kajii, S. Ikemoto, Y. Amemiya, N. Komatsu, T. Suda, Y. Miura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In order to confirm erythroid engraftment, we examined the changes in blood group antigens expressed on erythroblasts in the haematopoietic colonies of a bone marrow transplant recipient. A 44-year-old female with acute myelogenous leukaemia underwent an allogeneic bone marrow transplantation from her sister donor. Prior to the transplantation, the blood groups of both recipient and donor were analysed, and their M/N groups found to differ, the former being MN and the latter N. Bone marrow mononuclear cells were obtained from the patient on day 21 after bone marrow transplantation and cultured in semi-solid medium. Erythroblasts were collected from the erythroid bursts that had formed, and were subjected to flow cytometry using monoclonal anti-M and anti-N. Anti-N reactive cells accounted for 99.2% of those in the erythroid bursts, while only 0.3% of these cells were anti-M reactive. MN type erythrocytes in the recipient's peripheral blood had been replaced by N type and only 2.0% of erythrocytes were anti-M reactive on the 70th day after BMT. These erythoblasts and erythrocytes were phenotypically N, and originated from the donor haemopoietic stem cells with the success of bone marrow engraftment. From our findings, it appears that the M/N blood group antigens were produced by the erythroid cells themselves.

Original languageEnglish
Pages (from-to)329-333
Number of pages5
JournalBritish Journal of Haematology
Volume69
Issue number3
DOIs
Publication statusPublished - 1988 Jan 1

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Confirmation of bone marrow engraftment by detection of M/N blood group antigens on erythroblasts in erythroid bursts'. Together they form a unique fingerprint.

  • Cite this