Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter

Y. Abe; K. Kamiya; T. Osaki; R. Kawano; N. Miki; S. Takeuchi

doi:10.1109/Transducers.2013.6626791

Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This paper proposes a confocal laser scanning microscopic (CLSM) observation system for liposomes and cells that are mechanically trapped on a parylene microfilter. CLSM allowed us to acquire three-dimensional and highly sensitive fluorescent images, which we exploited to evaluate the deformability of liposomes with or without cholesterol, the amount of calcein that was introduced into the liposomes via membrane proteins, and the contact areas of the adjacent cells. The proposed system is readily applicable to analyze a single liposome and cells.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	416-417
Number of pages	2
DOIs	https://doi.org/10.1109/Transducers.2013.6626791
Publication status	Published - 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: 2013 Jun 16 → 2013 Jun 20

Publication series

Name	2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Other

Other	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	13/6/16 → 13/6/20

Keywords

Confocal laser scanning microscopy
cell
liposome
microfluidics device
parylene

ASJC Scopus subject areas

Hardware and Architecture
Electrical and Electronic Engineering

Access to Document

10.1109/Transducers.2013.6626791

Cite this

Abe, Y., Kamiya, K., Osaki, T., Kawano, R., Miki, N., & Takeuchi, S. (2013). Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 416-417). [6626791] (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6626791

Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter. / Abe, Y.; Kamiya, K.; Osaki, T. et al.

2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 416-417 6626791 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abe, Y, Kamiya, K, Osaki, T, Kawano, R, Miki, N & Takeuchi, S 2013, Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6626791, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, pp. 416-417, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 13/6/16. https://doi.org/10.1109/Transducers.2013.6626791

Abe Y, Kamiya K, Osaki T, Kawano R, Miki N, Takeuchi S. Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 416-417. 6626791. (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). doi: 10.1109/Transducers.2013.6626791

Abe, Y. ; Kamiya, K. ; Osaki, T. et al. / Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 416-417 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

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abstract = "This paper proposes a confocal laser scanning microscopic (CLSM) observation system for liposomes and cells that are mechanically trapped on a parylene microfilter. CLSM allowed us to acquire three-dimensional and highly sensitive fluorescent images, which we exploited to evaluate the deformability of liposomes with or without cholesterol, the amount of calcein that was introduced into the liposomes via membrane proteins, and the contact areas of the adjacent cells. The proposed system is readily applicable to analyze a single liposome and cells.",

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Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter

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