Confocal laser scanning microscopic observation of deformation, biological reaction, and contact of cells using mechanical trapping system with parylene micro filter

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, N. Miki, S. Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper proposes a confocal laser scanning microscopic (CLSM) observation system for liposomes and cells that are mechanically trapped on a parylene microfilter. CLSM allowed us to acquire three-dimensional and highly sensitive fluorescent images, which we exploited to evaluate the deformability of liposomes with or without cholesterol, the amount of calcein that was introduced into the liposomes via membrane proteins, and the contact areas of the adjacent cells. The proposed system is readily applicable to analyze a single liposome and cells.

Original languageEnglish
Title of host publication2013 Transducers and Eurosensors XXVII
Subtitle of host publicationThe 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages416-417
Number of pages2
DOIs
Publication statusPublished - 2013
Event2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain
Duration: 2013 Jun 162013 Jun 20

Publication series

Name2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Other

Other2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
CountrySpain
CityBarcelona
Period13/6/1613/6/20

Keywords

  • Confocal laser scanning microscopy
  • cell
  • liposome
  • microfluidics device
  • parylene

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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