Conformational epitope mapping and IgG subclass distribution of desmoglein 3 in paraneoplastic pemphigus

Yuko Futei, Masayuki Amagai, Takashi Hashimoto, Takeji Nishikawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Pemphigus vulgaris (PV) shows autoimmune reaction against desmoglein 3 (Dsg3), whereas paraneoplastic pemphigus (PNP) shows autoimmune reaction against Dsg3 as well as numerous members of the plakin family. It has been demonstrated that in PV, dominant epitopes reside in N-terminal adhesive regions of Dsg3 and that the dominant IgG subclass against Dsg3 is IgG4. Objective: We attempted to map conformational epitopes and analyze IgG subclass distribution against Dsg3 in PNP. Method: Epitopes on Dsg3 for circulating IgG autoantibodies from PNP (n = 22) were studied with competition enzyme-linked immunosorbent assay (ELISA) using domain-swapped molecules between Dsg3 and Dsg1 and were compared with those for IgG autoantibodies from PV (n = 22). IgG subclass distribution was analyzed with PNP serum by Dsg3 ELISA (n = 17). Results: Epitopes on Dsg3 in PNP were distributed more broadly through the extracellular domain of Dsg3 than were those in PV, although the N-terminal extracellular domains of Dsg3 were more frequently recognized than the C-terminal extracellular domains. IgG subclass in PNP was IgG1 and IgG2 dominant. Conclusion: Autoimmune response against Dsg3 in PNP is more diversified than that in PV, a finding that suggests PNP and PV have different pathophysiologic mechanisms for triggering production of anti-Dsg3 IgG.

Original languageEnglish
Pages (from-to)1023-1028
Number of pages6
JournalJournal of the American Academy of Dermatology
Volume49
Issue number6
DOIs
Publication statusPublished - 2003 Dec

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Desmoglein 3
Epitope Mapping
Pemphigus
Immunoglobulin G
Epitopes
Autoantibodies
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Dermatology

Cite this

Conformational epitope mapping and IgG subclass distribution of desmoglein 3 in paraneoplastic pemphigus. / Futei, Yuko; Amagai, Masayuki; Hashimoto, Takashi; Nishikawa, Takeji.

In: Journal of the American Academy of Dermatology, Vol. 49, No. 6, 12.2003, p. 1023-1028.

Research output: Contribution to journalArticle

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N2 - Background: Pemphigus vulgaris (PV) shows autoimmune reaction against desmoglein 3 (Dsg3), whereas paraneoplastic pemphigus (PNP) shows autoimmune reaction against Dsg3 as well as numerous members of the plakin family. It has been demonstrated that in PV, dominant epitopes reside in N-terminal adhesive regions of Dsg3 and that the dominant IgG subclass against Dsg3 is IgG4. Objective: We attempted to map conformational epitopes and analyze IgG subclass distribution against Dsg3 in PNP. Method: Epitopes on Dsg3 for circulating IgG autoantibodies from PNP (n = 22) were studied with competition enzyme-linked immunosorbent assay (ELISA) using domain-swapped molecules between Dsg3 and Dsg1 and were compared with those for IgG autoantibodies from PV (n = 22). IgG subclass distribution was analyzed with PNP serum by Dsg3 ELISA (n = 17). Results: Epitopes on Dsg3 in PNP were distributed more broadly through the extracellular domain of Dsg3 than were those in PV, although the N-terminal extracellular domains of Dsg3 were more frequently recognized than the C-terminal extracellular domains. IgG subclass in PNP was IgG1 and IgG2 dominant. Conclusion: Autoimmune response against Dsg3 in PNP is more diversified than that in PV, a finding that suggests PNP and PV have different pathophysiologic mechanisms for triggering production of anti-Dsg3 IgG.

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