Congenital osteoclast deficiency in osteopetrotic (op/op) mice is cured by injections of macrophage colony-stimulating factor

Hiroaki Kodama, Akira Yamasaki, Makoto Nose, Shumpei Niida, Yasuhisa Ohgame, Moriaki Abe, Masayoshi Kumegawa, Toshio Suda

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Abstract

Osteopetrotic (op/op) mice have a severe deficiency of osteoclasts, monocytes, and peritoneal macrophages because of a defect in the production of functional macrophage colony-stimulating factor (M-CSF) resulting from a mutation within the M-CSF gene. In this study, we examined whether daily 5-μg injections of purified recombinant human M-CSF (rhM-CSF) for 14 d would cure these deficiencies in the mutant mice. Monocytes in the peripheral blood of the op/op mice were significantly increased in number after subcutaneous injections of the factor two or three times a day. In contrast, osteopetrosis in the long bones of op/op mice was completely cured by only one injection of rhM-CSF per day. Bone trabeculae in the diaphyses were removed. Many osteoclasts were detected on the surface of bone trabeculae in the metaphyses. Although development of tooth germs of uninjected op/op mice was impaired, rhM-CSF injection restored the development of molar tooth germs and led to tooth eruption as a consequence of the recovery of bone-resorbing activity. These results demonstrate that M-CSF is one of the factors responsible for the differentiation of osteoclasts and monocyte/macrophages under physiological conditions.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalJournal of Experimental Medicine
Volume173
Issue number1
Publication statusPublished - 1991

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Macrophage Colony-Stimulating Factor
Osteoclasts
Injections
Tooth Germ
Monocytes
Bone and Bones
RANK Ligand
Osteopetrosis
Tooth Eruption
Diaphyses
Peritoneal Macrophages
Subcutaneous Injections
Macrophages
Mutation
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Kodama, H., Yamasaki, A., Nose, M., Niida, S., Ohgame, Y., Abe, M., ... Suda, T. (1991). Congenital osteoclast deficiency in osteopetrotic (op/op) mice is cured by injections of macrophage colony-stimulating factor. Journal of Experimental Medicine, 173(1), 269-272.

Congenital osteoclast deficiency in osteopetrotic (op/op) mice is cured by injections of macrophage colony-stimulating factor. / Kodama, Hiroaki; Yamasaki, Akira; Nose, Makoto; Niida, Shumpei; Ohgame, Yasuhisa; Abe, Moriaki; Kumegawa, Masayoshi; Suda, Toshio.

In: Journal of Experimental Medicine, Vol. 173, No. 1, 1991, p. 269-272.

Research output: Contribution to journalArticle

Kodama, H, Yamasaki, A, Nose, M, Niida, S, Ohgame, Y, Abe, M, Kumegawa, M & Suda, T 1991, 'Congenital osteoclast deficiency in osteopetrotic (op/op) mice is cured by injections of macrophage colony-stimulating factor', Journal of Experimental Medicine, vol. 173, no. 1, pp. 269-272.
Kodama, Hiroaki ; Yamasaki, Akira ; Nose, Makoto ; Niida, Shumpei ; Ohgame, Yasuhisa ; Abe, Moriaki ; Kumegawa, Masayoshi ; Suda, Toshio. / Congenital osteoclast deficiency in osteopetrotic (op/op) mice is cured by injections of macrophage colony-stimulating factor. In: Journal of Experimental Medicine. 1991 ; Vol. 173, No. 1. pp. 269-272.
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