Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

Mitsuhiro Itaya; Nagayoshi Sakaya; Satoko Matsunaga; Kyoko Fujita; Shinya Kaneko

doi:10.1271/bbb.70.740

Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

Mitsuhiro Itaya, Nagayoshi Sakaya, Satoko Matsunaga, Kyoko Fujita, Shinya Kaneko

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

Original language	English
Pages (from-to)	740-742
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	70
Issue number	3
DOIs	https://doi.org/10.1271/bbb.70.740
Publication status	Published - 2006
Externally published	Yes

Keywords

Bacillus subtilis
Conjugational transfer
pLS20

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1271/bbb.70.740

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abstract = "pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.",

keywords = "Bacillus subtilis, Conjugational transfer, pLS20",

author = "Mitsuhiro Itaya and Nagayoshi Sakaya and Satoko Matsunaga and Kyoko Fujita and Shinya Kaneko",

note = "Funding Information: We are thankful to Dr. K. Tsuge for construction of the recA strain. This work was supported by a research grant (No. 16380066) from the Ministry of Education, Culture, Sports, Science and Technology.",

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language = "English",

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AU - Itaya, Mitsuhiro

AU - Sakaya, Nagayoshi

AU - Matsunaga, Satoko

AU - Fujita, Kyoko

AU - Kaneko, Shinya

N1 - Funding Information: We are thankful to Dr. K. Tsuge for construction of the recA strain. This work was supported by a research grant (No. 16380066) from the Ministry of Education, Culture, Sports, Science and Technology.

PY - 2006

Y1 - 2006

N2 - pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

AB - pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

KW - Bacillus subtilis

KW - Conjugational transfer

KW - pLS20

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 3

ER -

Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

Abstract

Keywords

ASJC Scopus subject areas

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