Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

Mitsuhiro Itaya, Nagayoshi Sakaya, Satoko Matsunaga, Kyoko Fujita, Shinya Kaneko

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

Original languageEnglish
Pages (from-to)740-742
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number3
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Bacilli
Bacillus subtilis
developmental stages
mutation
kinetics
Mutation
Kinetics
liquids
Liquids
Growth

Keywords

  • Bacillus subtilis
  • Conjugational transfer
  • pLS20

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium. / Itaya, Mitsuhiro; Sakaya, Nagayoshi; Matsunaga, Satoko; Fujita, Kyoko; Kaneko, Shinya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 3, 2006, p. 740-742.

Research output: Contribution to journalArticle

Itaya, Mitsuhiro ; Sakaya, Nagayoshi ; Matsunaga, Satoko ; Fujita, Kyoko ; Kaneko, Shinya. / Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 3. pp. 740-742.
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