Conjugational transfer kinetics of pLS20 between Bacillus subtilis in liquid medium

Mitsuhiro Itaya, Nagayoshi Sakaya, Satoko Matsunaga, Kyoko Fujita, Shinya Kaneko

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

pLS20-mediated conjugational transfer between Bacillus subtilis was investigated not on conventional solid media but in liquid culture. Detailed conjugational kinetics revealed that pLS20 transmission occurred at a limited cellular growth stage of both donor and recipient Mutation of the recipient recA did not significantly interfere with the conjugational transfer process.

Original languageEnglish
Pages (from-to)740-742
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number3
DOIs
Publication statusPublished - 2006

Keywords

  • Bacillus subtilis
  • Conjugational transfer
  • pLS20

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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